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8.5 dpc

DEV0177ER Fig. S2 Atchison et al. anti-Pin1 alkaline phosphatase pre-absorbed anti-Pin1. C. 8.5 dpc. B. A. 7.5 dpc. F. E. D.

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8.5 dpc

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  1. DEV0177ER Fig. S2Atchison et al. anti-Pin1 alkaline phosphatase pre-absorbed anti-Pin1 C 8.5 dpc B A 7.5 dpc F E D Immunostaining for Pin1 (green) in 7.5 (D) and late 8.5 dpc (A) embryo sections show that Pin1 is expressed in PGCs and somatic cells in those stages. Adjacent sections (B, E) were stained for alkaline phosphatase (brown) to reveal the areas containing PGCs (arrows). (C, F) Negative controls using anti-Pin1 antibodies pre-absorbed with Pin1 protein show the specificity of the Pin1 staining. Red in (A, C, D, F), DAPI stained nuclei.

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