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I. Introduction to PTMs Protein Phosphorylation (Ser/Thr) Methods ―enrichment. Applications

Lec 4 Post-Translational Modifications (PTMs). ― Any covalent modification to a protein after translation: (reversible and non-reversible). I. Introduction to PTMs Protein Phosphorylation (Ser/Thr) Methods ―enrichment. Applications Membrane “shaving” Label-free quantitative analysis

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I. Introduction to PTMs Protein Phosphorylation (Ser/Thr) Methods ―enrichment. Applications

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  1. Lec 4 Post-Translational Modifications (PTMs) ―Any covalent modification to a protein after translation: (reversible and non-reversible) • I. Introduction to PTMs • Protein Phosphorylation (Ser/Thr) • Methods―enrichment. • Applications • Membrane “shaving” • Label-free quantitative analysis • Large-scale phosphorylation site mapping • pS/pT-binding proteins (intro to Pro:Pro)

  2. TIBS (2007) 34: 108

  3. Proteomic analysis of PTMs Mann and Jensen, Nature Biotech. 21, 255 (2003)

  4. Reversible Protein Phosphorylation Regulates Most Aspects of Cell Life 1992 Nobel Prize to Fischer and Krebs

  5. INFORMATION Protein Second Protein Synthesis Messengers Phosphorylation PEPC kinase CDPKs MAPKs SnRK1s OGT OGase Reversible Protein Phosphorylation OGT = O-GlcNAc Transferase, encoded by SPY

  6. Protein Phosphorylation • One of the most common intracellular protein modification • Approximately 5 % of the Arabidopsis thaliana genome encodes for protein kinases & phosphatases (H. sapiens: 5 %) • Approximately 30 % of all human proteins are phosphorylated. How about plants, e.g., A. thaliana? • Protein phosphorylation is of special importance in the regulation of functions, e.g. metabolism or cell signaling In order to better understand the molecular mechanisms where phosphorylation is involved this modification has to be analyzed.

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