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MLAB 1415: Hematology

MLAB 1415: Hematology. Micros 60. Micros 60. 3-part differential Sample size= 10µL Environment Indoor only Level work station Avoid direct exposure to sunlight Proper ventilation 60-90 o F Linearity (AMR) WBC (10 3 /mm 3 ): 0-100 RBC (10 6 /mm 3 ): 0-8.0 Hgb (g/ dL ): 2.0-27

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MLAB 1415: Hematology

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  1. MLAB 1415: Hematology Micros 60

  2. Micros 60 • 3-part differential • Sample size= 10µL • Environment • Indoor only • Level work station • Avoid direct exposure to sunlight • Proper ventilation • 60-90oF • Linearity (AMR) • WBC (103/mm3): 0-100 • RBC (106/mm3): 0-8.0 • Hgb (g/dL): 2.0-27 • Hct (%): 1.8-82.3 • Plt (103/mm3): 25-4990

  3. Parameters Parameters Measurements/Calculations WBC/RBC/PLT Impedance change HGB Spectrophotometry LYM%/MON%/GRA% Impedance change Calculations MCV, MCH,MCHC,RDW,MPV,LYM#, MON#, GRA# • WBC • RBC • Hgb • Hct • MCV • MCH • MCHC • RDW • Platelet • MPV • Lym # • Lym% • Mono # • Mono% • Gra# • Gra%

  4. Stats

  5. Equipement • 8 Main parts • Electrical supply • Electronic board • Dilution pneumatics • Control panel with keyboard and LCD screen • Reagent compartment • Printer • Smart card reader • Bar code reader (optional)

  6. Reagents • Diluent • ABX minidil: sodium hydroxide/azide • Determines and differenciates blood cells • Cleaner • ABX miniclean: enzymatic detergent • Cleans system • LYSE: potassium cyanide • ABX Alphalyse: Lyses RBCs, releasing hemoglobin and WBCs. WBCs are counted by impedance. • Waste

  7. Distribution curves • WBC • RBC • Plt

  8. Daily Start Up • Turn on instrument, once on, the start-up will begin automatically • Instrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluent • Cycle lasts about 130 seconds • At cycle completion, background values will be displayed. These values must be less than the below: • WBC < 0.3 x 103/mm3 • RBC < 0.02 x 106/mm3 • Hgb< 0.0 g/dL • Plt< 10x 103/ mm3 • If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.

  9. Daily start-up con’t • Run a primer sample • Perform QC and evaluate • Complete maintenance sheet • If all QC in, can continue with patient samples.

  10. Running a Patient • Press the ID button on the key pad • Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.

  11. Shutdown • Hit the Stand by key, wait 1 minute • Switch off instrument and printer.

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