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DNA – What is it?. A Rather Large Molecule That Encodes for Genetic Instruction. * Deoxyribonucleic acid. ** DNA is a polymer in the nuclei of cells. **Made up of monomer subunits called nucleotides .
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A Rather Large Molecule That Encodes for Genetic Instruction * Deoxyribonucleic acid ** DNA is a polymerin the nuclei of cells **Made up of monomer subunits called nucleotides * Most of your cells have identical DNA but genes are “turned on” according to what proteins are made *Example: skin cells will not produce proteins for the liver
Nucleotides **DNA Nucleotides float in the cytoplasm and have three parts: The Backbone Nitrogenous base Phosphate group **There are 4 nitrogen-containing bases: Sugar (deoxyribose) *Adenine (A) *Thymine (T) *Cytosine (C) *Guanine (G)
For Simplicity Purposes – A Single Nucleotide A, G,T or C
Nitrogenous Bases Make Up The DNA “Alphabet” – A,G,T,C ALWAYS Pairs With Purines Pyrimidines ALWAYS Pairs With Cytosine (C) Guanine (G) Thymine (T) Adenine (A) ** Make notice that purines have 2 rings and pyrimidines have one. This is what will gives DNA molecules their shape!!
DNA ** All of the rungs of the DNA “ladder “are pairs of nucleotides, coiled into the shape of a double helix!! **Like a spiral staircase **“rungs” are held together by hydrogen bonds Remember base-pairing rules!!! A with T C with G Nucleotides are held together by hydrogen bonds
Structure of DNA’s Nucleotides Proposed by Watson and Crick in 1953
Genes are Found on Chromosomes (We’ll Discuss These a Little Later!)
Genes • Made up of a series of DNA nucleotides • Only the 4 letters of DNA’s “alphabet” • All of your genes make up a genome • There are about 21, 000 genes • About 3.2 billion base pair • It is estimated that in one single human cell, if you uncoiled the double helix, it would be about 6 foot long!
Genes • Can be several hundred to several thousand base-pair long • Some genes carry the instructions to make proteins that give you traits, like eye color, hair color and freckles! • Some genes are regulatory genes that may control other genes • Some genes are promotor genes, sequences of DNA needed to turn genes “on” or “off”
On the Next Slide is an Example of Part of a Code for a Gene and is only one side of the “ladder”
GACAGACAGACAGACAGACGGACATGCTCATATCGACTCAGGAGGTGATCCTGATCAAGAATATATATACTTTATAGGGTCGGAGATGTCTTCTTCACTGCGTTGCACACTTTTGACCAAAATTATAATACCCTCTGCAAGGGTATAAAAATGAAGCTATACTATATAGTTATTATATATATGCAAAGTCTGGACAAAATTTTGTGCCGATCGGGCCACCACTTTCTTTTTGCCCCATAGAATCCAATGCTCCCTGGGACAAAAATTTACAAAATATTCTTGAAAATTGGGTCAATATATCTAATAAATTATAATCCGGCACTATCCAACCTACTTTTATTCCATGAAAAATGCTAGGTTGAAATTGTTTAATATTCAACTTTACAATATGTATATGCGCTTATACACAAATACACATCACATTAAACAAATATGGAATTAAACACTTAAATTTTACATACCTACACTAATAATTTCCATTTTACGTTGTTTTAAAATATCCAGTATAACATTTGAAATCTTACTTTAAAATGTGATATTATTTAGCGCGCCAAAAAGTCAACGTTTCCGATCAACGAACAGCTGACTTGGGGCATTGGGACCAGTGTGATGTCGAAGGAATCTCACATTAATACTCTGCATAAAAAAGGGAAAAAATCTTACGCTGCCAATTATAGGGGCATTTCTAAATTGTCAGCAAGCCTTCTTGAAAAACGAATTACTCCACATTTTAAACACCTTTATAGTTCAATAATATGATCGTGCCAGCATGCCTTCATGAAGAGCCACTCTACCACTACCAACTTTTTGGAGCTAACATCTTTTGTCACGGATGGGTTCCATTTACACTGACTTTAGTTAAGATTTTGACTCTGTTAACCATCCTACATTTTACATTGTGTATTAACGACTTTCCCCTTGTTTTAACCAATTCACTTTTACTTATGTATATCTGCATTATGGATTCACTTGTGCCGAATCTAAACTTCAATCCGATTTGGCCAGATATTAAAATTGGTGTTTTGAAAATAACCTAAGACTTATTGTATGCAACTGTAAATTCGTGTCATTTTATCGATTTAGAGCAGGGATGGCCCTGTTGTTAGATTGCTCTGTCATATGGGAAGAGCAATGTCATATACGTGTGCTGCTCCCAGTATAGGCGATGAAAATTAATAAAAAAAAATTTTGCGTGGCTCAACAACGTTTAACATAGTTTCATAGTTAACTTAACTTCTTTGGTCTGTCCGATATTTAAGTATCGTGTAGTAGAGCCCTCATTATGGAGTAAACCGAGATCACATCGAATCTGTACAAAAAAATTCCTGATTTTCCTGACGATATTTCCAACATACATATATCTAATAATATAAACTGCCAACTAACTGCAAGCCGGTAAATATCAACTTCGCCTCCGCCCGATGTGGAGTTTTCTTTCTTGTTATAATTTGTTTACGTTAGAAATTAAAAAAATAATTTTTTAAAGAAAAAGATGGAAAGCTACAAAGCCACCACAAAGCCGCTTACAGAAAAGTGAAAAAAATTGTAAATGCCGTAAAAATTGAGAATATCAAGATGTCAATCAATGCGCATTCAATATTATAGTGTTATTAAAGATAAAGAAAGAAATACTTTTTTATAGATATGGTGACTATAACTTTCCAAAAATATAGTGAATGAACATTTTAATACTCCTTGAGCACATTAAGGCATGCGATACGACATTTCAATTCTTTCGAACCGAAGCGCATAAGTGCAAAAGGCAACAACCTTAAAAAGTCAACAACCTTGAAAGCAATTTGAATATTTGTATATAAATTAAATGATAAATGCCAAAAAAATATAACTTTGATCCACTTTCAAGAACGCTTTTACTTTTGTTTGATACTGTTTGTTACTAAGTGATTGTAACTAAATGATTCAACAAAAAGATATTAAAATTTCCATATTATAAGGGTGTGTCCAATTTTCGTGTTTTTAGCTTAATTTGAATTTCCAAATTAATTATACATACAAAATACTTACATTATTGCAATTTCTCATCTATTTACATTTCAATGTTAACCAATTCTTGAATTTACATGAAAAAGCCTATTTACACAGTTCACGATTTTAATTACTTTGGATTGTTTGTGGTCAAAGTATTTTAGGTATATTTTTTTGGAATTGATAATAAAGTCAGTTTTGTTGCAACCGCATATATAAACTCACACTCGAGTATTTACGGGTTAGTTGCCAATTGCTTAGCAATTCGTTAATACGCTTTCAATTTATTTAATGTTAACGCCGCAAACATATACACCGTTTTAAACATCAACGACTTTAAATAATTAGAAATTTAACAAAAACTTAAATGGAGATGGCAAAAAATAAGAAATATTCCCGGTCTCTGCCAGAATCAGACGGAGATGATATAGTAATTTCTGGAATGGCCGGAAAATTTCCAAACTGTAGCAACATATCGGAATACGAGTTTAAACTATATAACAAGGTTAGAAACAAAGTTTAAAATAAAACGTCCAATGGGGAAAACCATCTAATAATACTGATATGAAAAAAATTTGGTGAATAAAACATAACAAGAGCATTTTTAAACTGTGAATTGAAATAAAATAGTTGATTAAAAAGAACAGTACGCAGGAAATGGAAAAGTAAAAAACACAAGAAAGAAAAGATAACTTTGGGCGCAGCCTAAGTAGCAGAAATTTCTTGCGACTTGCCCCCAGAGGCCCCAGACAGAATCTCACTTCCCGTCCAATCGACCGAGGGACGCTGCACATAACGTACTCAAATCGTGGGGATAGATCCGAGAAAGTAGGAGTAAGATATCACGACCAAGAGAGTTGCGGATAAGGCGTTTAGTATAAGGGATTTAGAATTGTTAGTGGAGCAAAGGGACAGGATGTGGTAGAGATCATTGTAGTCCGAACATAATACCCTGAACGGATCGTGTTGGGCAAATGTCGAACTACAATGGCTGAAGAGTAGAGGACGAAAGATTCTAGTCCGCCTACTAGGAACGTTAAAATTTAAACGAGTTAGTAAATGCTAGCTGTCTTCATTACCATAAATAGGATTGTATAGAAACATAACGCTTTATTAATGACGGTAAGTTGATTAATAAAAGTCTACTAATATAAGAGAGGAGGTGGAGATTGGCATCCCAATTAAGTCCACTAAGGGCGAAAATCAGGAAATTTTTTTGAACTGATCTCGGTGTACTCCGTATTGAGGACTCCAGTGACACGAATCATTAAATTCTTTTGACCATCTTTTCATAAAACCAAATACACCCATTATTAACCAGTTATATTAACCATGGTGGATGTATGGTTGGTAAATTTAAGTTTCAAATTTAATATGACCTAGATCGTTACCTGAGTTAATCCTTCTAAGGCGTTCCCATAGAGGAAATACATAGCCTAATGATGACTAGATCGATAAAATGACATTAGTTTACATTTCGATCCATTAAGCGTTAGCTTATTTGCTAAACACCAATTTTGAAATTTATTCAAATAGGATTAAAGTCTAGATTGGGGCACCCAAGATGTAGCGCGCAATAAACGCGAGGTAACATTTTTAAAGAAGACACATTGGGTTCTTTAAGATAAGTAGCTCCAAATCCAGATAAGAAGGTCAGTTGGGAATCCCAAAAAAACTGAGTTTTCTTATAAGAAGCGAATTGTTAACAGAGAAAAATCCTTTACTGAAGTCGGTGTAAATGACGTCTGTTTGTAAGCCATTCCGGAACCAATCGTGACAAAAGATGTTAACTCCTATAAGTTGGTAGTTGCGGAGTGGCGCTTCATGAAGCCATGCTGGCACGGAGATATTACTTACAGAATTAACACTCCTCCTCCCTTTCGCAGGGGTCGACGTCAAAATGTACTTACTTGGAAAAAATTTAGAGCTTAAGATCTCCGGCTTTAACCAAGTTTATGTAAAGAACCTAATGTGAGATGCAAAGGAAGAACTATAAGAATACATTTTGGGAAGTTTACTACGTAACCCTGGAGTATTCTGATAGGTAAATGTTAGTAACAAAAATTAATTTTTTCGAATCTGATGAAGATGAGATTAAAGGTTGTTTAGTGGCTGTAACATCTGGCAATTTTACTCATACAGGTTTGGTCATTTTGTTTTTTTTGACGAAAATGTTCTCTGGCCAAGAACAGGCGAAACAAATCGTCTTGAATATCTGCAAGGGCACACGTATACCTGTGCCTGGTGTATGAATATTTAAATTTTGTAATATACACCACCTTTATGTCGGCTTTTGTTTTGGCTTTGATGTTAGCCGATATATCAATCTCAGAAGTATCAGAGGCAAGCTGGGAAATAAAAATGTGTTTCGATGGAGGAACCACCTGCAACAGTTTCGGTGCCGTCGTAACTAGTTCTGCGACATCAGTTCGTACCGGGGGTCCAGACTTTTGATTACCCTGTTCGGACTTCTCAGGGGTCTTGAATTATTGGGTCTGGAATCACTTGAAGCGATGCCAAAAAGGACATATCAGACAATTGCACATTTATTTTGAGATATTCGGAAGCCGAATTTTTCTCAGGTTCGGCCCTTGGGGTTGCCAGAGATATAAGCGGCTGCATAATCGTCGGTAAAAGAGGATTTCTTCGCTTTGGAGACTTATTTAGTAGTTGAAGACTACTAAACCGTATATCGAGCGCACAGAGTTGGTCATTAATTTTACGAAAACCGCTAATCAACTCGTTGAATCCACTTTTAGCACGACAACCGTCACATTACAAGTAGAGACCGGATCTACGTGAAATAGCATCCGGCACACTTAGCGTGTAAAACGTTTTCACACAGTGAAATGAAAAAGGGAGATTAAAGAGAGCGATTAGTGTGGGTTAGTAAACTGCAAAGAATAGAGATAAGAATCTCTATTGAGAGAGAATAGAAGCATAATCGTAAAAGATGAAGAAGCAGAGCAGGGCTATTTTTGAAAGAAAATTAATTAAGTTATTATTTTACCTCCAAATATATCACTGCACACGCGAAGCAATACGGATGTTTGCGAATGCGAAAGTTTGTTTTTATAAATGTAAATAAATAAAAATAAACACTGGATTGTTAGCTAATAAAAGATTTGATAATAACGCACAAAAATCCGCTTTCAGATAAAGAGAGGCTGTCCTTTTCATAAAAAGATTTGTATTTTATCATACGTTTTTAAAAATTAACGCATTTTACCAAATATTATTATAATTATTAATTAATTTTTAACTTTTAAGATAGATATGGTTGACGATGATGAGCGCAGATGGCGTCATTTTAACCCGGAAATACCAAAACGATCTGGAAAAATTAGTGAGCTTGAAAAATTTGATGCAACATTTTTTGGAGTTCATTTTAAGCAAGCTCATACAATGGATCCCCAAACTCGAATTTTGATTGAGACTGCATATGAAGCGGTCATAGATTCCGGAATAAACCCAAAAAGTCTTCGGGGATCAAAAACTGGAGTGTACATTGGATCTTGTATTTCTGAATCAGAAAAAACATGGTTTTATGAAAAAGTTTCATCTGGTGGATTTGGAATAACTGGCTGCAGTCGGGCTATGATGGCTAATAGAATATCTTACTGTTTAGGACTCGAGGGACCTTCTTTTCTATTAGATACTGCATGTTCAAGTTCAATGTACGCGTTGGATAATGCCTTTAGTGCACTTCGAAATGGAGAAATAGATGCAGCAATAATTGGTGGCTCCAACTTACTTCTTCATCCATTTGTGACTCTTCAATTTGCTCGGTAAGCTTTTTGATTTTTTTAAAATTTTATTTCCTAACAAACTTTAAATTCCATAACATCATAGACTCGGCGTACTTGCTCCAGATGGGTTTTGTCGTCCATTCGATAAAAATGCAAGTGGCTATACCAGATCTGAAACTATCAATTGCCTATTTTTGCAACGAAAACGGGATGCAAAGCGTGTGTATGCTAGTGTTGTGTATTCAAAAACTAATTGTGATGGATATAAGCCAGAGGGTATAACGTATCCATCTGGGGTAGTCCAAGAAAAGTTGTTGGAAAATTTCTATAAAGAGATCGAACTTACCCCCTCAGATTTGGGCTATTTAGAGGCTCACAGTACTGGAACTGTAGTTGGTGATCCAGAAGAATGCAAAGCAATAGACAATGTAATTTGTAGCCAAAGACGCGAGCCTTTATTAATTGGATCTGTTAAATCAAATATTGGACATTCCGAAGCTGCATCAGGAATATGCTCACTTGTTAAAGCTTGCTTTGCATTTGAAACTGGCATGATTGCACCGAATATAAATTTTGCAGAAGTAAAACCAATTATTGCACCTCTAGCAGAAGGAAGATTGCTTGTTGTTAAAGATAAAACGCCTCTACCAAAGCCATACATCGGTATAAATTCTTTTGGATTTGGAGGTGCAAATGCACATGCACTTCTGAAAGGTTTTACCAAATCAAAGACAAATTTTGGGATTCCTGAGGATGATGTACCTCGACTTCTTACATGGGCCGGTAGGACAGAAGATGCAGTGCATGAAATTTTTAGCGACATAGAAAAAAGACCCCTAGATGCAGAATTTATCGGACTACTTCAAAATATTCAAGAGGGACAGACAGACAGACAGACGGACATGCTCATATCGACTCAGGAGGTGATCCTGATCAAGAATATATATACTTTATAGGGTCGGAGATGTCTTCTTCACTGCGTTGCACACTTTTGACCAAAATTATAATACCCTCTGCAAGGGTATAAAAATGAAGCTATACTATATAGTTATTATATATATGCAAAGTCTGGACAAAATTTTGTGCCGATCGGGCCACCACTTTCTTTTTGCCCCATAGAATCCAATGCTCCCTGGGACAAAAATTTACAAAATATTCTTGAAAATTGGGTCAATATATCTAATAAATTATAATCCGGCACTATCCAACCTACTTTTATTCCATGAAAAATGCTAGGTTGAAATTGTTTAATATTCAACTTTACAATATGTATATGCGCTTATACACAAATACACATCACATTAAACAAATATGGAATTAAACACTTAAATTTTACATACCTACACTAATAATTTCCATTTTACGTTGTTTTAAAATATCCAGTATAACATTTGAAATCTTACTTTAAAATGTGATATTATTTAGCGCGCCAAAAAGTCAACGTTTCCGATCAACGAACAGCTGACTTGGGGCATTGGGACCAGTGTGATGTCGAAGGAATCTCACATTAATACTCTGCATAAAAAAGGGAAAAAATCTTACGCTGCCAATTATAGGGGCATTTCTAAATTGTCAGCAAGCCTTCTTGAAAAACGAATTACTCCACATTTTAAACACCTTTATAGTTCAATAATATGATCGTGCCAGCATGCCTTCATGAAGAGCCACTCTACCACTACCAACTTTTTGGAGCTAACATCTTTTGTCACGGATGGGTTCCATTTACACTGACTTTAGTTAAGATTTTGACTCTGTTAACCATCCTACATTTTACATTGTGTATTAACGACTTTCCCCTTGTTTTAACCAATTCACTTTTACTTATGTATATCTGCATTATGGATTCACTTGTGCCGAATCTAAACTTCAATCCGATTTGGCCAGATATTAAAATTGGTGTTTTGAAAATAACCTAAGACTTATTGTATGCAACTGTAAATTCGTGTCATTTTATCGATTTAGAGCAGGGATGGCCCTGTTGTTAGATTGCTCTGTCATATGGGAAGAGCAATGTCATATACGTGTGCTGCTCCCAGTATAGGCGATGAAAATTAATAAAAAAAAATTTTGCGTGGCTCAACAACGTTTAACATAGTTTCATAGTTAACTTAACTTCTTTGGTCTGTCCGATATTTAAGTATCGTGTAGTAGAGCCCTCATTATGGAGTAAACCGAGATCACATCGAATCTGTACAAAAAAATTCCTGATTTTCCTGACGATATTTCCAACATACATATATCTAATAATATAAACTGCCAACTAACTGCAAGCCGGTAAATATCAACTTCGCCTCCGCCCGATGTGGAGTTTTCTTTCTTGTTATAATTTGTTTACGTTAGAAATTAAAAAAATAATTTTTTAAAGAAAAAGATGGAAAGCTACAAAGCCACCACAAAGCCGCTTACAGAAAAGTGAAAAAAATTGTAAATGCCGTAAAAATTGAGAATATCAAGATGTCAATCAATGCGCATTCAATATTATAGTGTTATTAAAGATAAAGAAAGAAATACTTTTTTATAGATATGGTGACTATAACTTTCCAAAAATATAGTGAATGAACATTTTAATACTCCTTGAGCACATTAAGGCATGCGATACGACATTTCAATTCTTTCGAACCGAAGCGCATAAGTGCAAAAGGCAACAACCTTAAAAAGTCAACAACCTTGAAAGCAATTTGAATATTTGTATATAAATTAAATGATAAATGCCAAAAAAATATAACTTTGATCCACTTTCAAGAACGCTTTTACTTTTGTTTGATACTGTTTGTTACTAAGTGATTGTAACTAAATGATTCAACAAAAAGATATTAAAATTTCCATATTATAAGGGTGTGTCCAATTTTCGTGTTTTTAGCTTAATTTGAATTTCCAAATTAATTATACATACAAAATACTTACATTATTGCAATTTCTCATCTATTTACATTTCAATGTTAACCAATTCTTGAATTTACATGAAAAAGCCTATTTACACAGTTCACGATTTTAATTACTTTGGATTGTTTGTGGTCAAAGTATTTTAGGTATATTTTTTTGGAATTGATAATAAAGTCAGTTTTGTTGCAACCGCATATATAAACTCACACTCGAGTATTTACGGGTTAGTTGCCAATTGCTTAGCAATTCGTTAATACGCTTTCAATTTATTTAATGTTAACGCCGCAAACATATACACCGTTTTAAACATCAACGACTTTAAATAATTAGAAATTTAACAAAAACTTAAATGGAGATGGCAAAAAATAAGAAATATTCCCGGTCTCTGCCAGAATCAGACGGAGATGATATAGTAATTTCTGGAATGGCCGGAAAATTTCCAAACTGTAGCAACATATCGGAATACGAGTTTAAACTATATAACAAGGTTAGAAACAAAGTTTAAAATAAAACGTCCAATGGGGAAAACCATCTAATAATACTGATATGAAAAAAATTTGGTGAATAAAACATAACAAGAGCATTTTTAAACTGTGAATTGAAATAAAATAGTTGATTAAAAAGAACAGTACGCAGGAAATGGAAAAGTAAAAAACACAAGAAAGAAAAGATAACTTTGGGCGCAGCCTAAGTAGCAGAAATTTCTTGCGACTTGCCCCCAGAGGCCCCAGACAGAATCTCACTTCCCGTCCAATCGACCGAGGGACGCTGCACATAACGTACTCAAATCGTGGGGATAGATCCGAGAAAGTAGGAGTAAGATATCACGACCAAGAGAGTTGCGGATAAGGCGTTTAGTATAAGGGATTTAGAATTGTTAGTGGAGCAAAGGGACAGGATGTGGTAGAGATCATTGTAGTCCGAACATAATACCCTGAACGGATCGTGTTGGGCAAATGTCGAACTACAATGGCTGAAGAGTAGAGGACGAAAGATTCTAGTCCGCCTACTAGGAACGTTAAAATTTAAACGAGTTAGTAAATGCTAGCTGTCTTCATTACCATAAATAGGATTGTATAGAAACATAACGCTTTATTAATGACGGTAAGTTGATTAATAAAAGTCTACTAATATAAGAGAGGAGGTGGAGATTGGCATCCCAATTAAGTCCACTAAGGGCGAAAATCAGGAAATTTTTTTGAACTGATCTCGGTGTACTCCGTATTGAGGACTCCAGTGACACGAATCATTAAATTCTTTTGACCATCTTTTCATAAAACCAAATACACCCATTATTAACCAGTTATATTAACCATGGTGGATGTATGGTTGGTAAATTTAAGTTTCAAATTTAATATGACCTAGATCGTTACCTGAGTTAATCCTTCTAAGGCGTTCCCATAGAGGAAATACATAGCCTAATGATGACTAGATCGATAAAATGACATTAGTTTACATTTCGATCCATTAAGCGTTAGCTTATTTGCTAAACACCAATTTTGAAATTTATTCAAATAGGATTAAAGTCTAGATTGGGGCACCCAAGATGTAGCGCGCAATAAACGCGAGGTAACATTTTTAAAGAAGACACATTGGGTTCTTTAAGATAAGTAGCTCCAAATCCAGATAAGAAGGTCAGTTGGGAATCCCAAAAAAACTGAGTTTTCTTATAAGAAGCGAATTGTTAACAGAGAAAAATCCTTTACTGAAGTCGGTGTAAATGACGTCTGTTTGTAAGCCATTCCGGAACCAATCGTGACAAAAGATGTTAACTCCTATAAGTTGGTAGTTGCGGAGTGGCGCTTCATGAAGCCATGCTGGCACGGAGATATTACTTACAGAATTAACACTCCTCCTCCCTTTCGCAGGGGTCGACGTCAAAATGTACTTACTTGGAAAAAATTTAGAGCTTAAGATCTCCGGCTTTAACCAAGTTTATGTAAAGAACCTAATGTGAGATGCAAAGGAAGAACTATAAGAATACATTTTGGGAAGTTTACTACGTAACCCTGGAGTATTCTGATAGGTAAATGTTAGTAACAAAAATTAATTTTTTCGAATCTGATGAAGATGAGATTAAAGGTTGTTTAGTGGCTGTAACATCTGGCAATTTTACTCATACAGGTTTGGTCATTTTGTTTTTTTTGACGAAAATGTTCTCTGGCCAAGAACAGGCGAAACAAATCGTCTTGAATATCTGCAAGGGCACACGTATACCTGTGCCTGGTGTATGAATATTTAAATTTTGTAATATACACCACCTTTATGTCGGCTTTTGTTTTGGCTTTGATGTTAGCCGATATATCAATCTCAGAAGTATCAGAGGCAAGCTGGGAAATAAAAATGTGTTTCGATGGAGGAACCACCTGCAACAGTTTCGGTGCCGTCGTAACTAGTTCTGCGACATCAGTTCGTACCGGGGGTCCAGACTTTTGATTACCCTGTTCGGACTTCTCAGGGGTCTTGAATTATTGGGTCTGGAATCACTTGAAGCGATGCCAAAAAGGACATATCAGACAATTGCACATTTATTTTGAGATATTCGGAAGCCGAATTTTTCTCAGGTTCGGCCCTTGGGGTTGCCAGAGATATAAGCGGCTGCATAATCGTCGGTAAAAGAGGATTTCTTCGCTTTGGAGACTTATTTAGTAGTTGAAGACTACTAAACCGTATATCGAGCGCACAGAGTTGGTCATTAATTTTACGAAAACCGCTAATCAACTCGTTGAATCCACTTTTAGCACGACAACCGTCACATTACAAGTAGAGACCGGATCTACGTGAAATAGCATCCGGCACACTTAGCGTGTAAAACGTTTTCACACAGTGAAATGAAAAAGGGAGATTAAAGAGAGCGATTAGTGTGGGTTAGTAAACTGCAAAGAATAGAGATAAGAATCTCTATTGAGAGAGAATAGAAGCATAATCGTAAAAGATGAAGAAGCAGAGCAGGGCTATTTTTGAAAGAAAATTAATTAAGTTATTATTTTACCTCCAAATATATCACTGCACACGCGAAGCAATACGGATGTTTGCGAATGCGAAAGTTTGTTTTTATAAATGTAAATAAATAAAAATAAACACTGGATTGTTAGCTAATAAAAGATTTGATAATAACGCACAAAAATCCGCTTTCAGATAAAGAGAGGCTGTCCTTTTCATAAAAAGATTTGTATTTTATCATACGTTTTTAAAAATTAACGCATTTTACCAAATATTATTATAATTATTAATTAATTTTTAACTTTTAAGATAGATATGGTTGACGATGATGAGCGCAGATGGCGTCATTTTAACCCGGAAATACCAAAACGATCTGGAAAAATTAGTGAGCTTGAAAAATTTGATGCAACATTTTTTGGAGTTCATTTTAAGCAAGCTCATACAATGGATCCCCAAACTCGAATTTTGATTGAGACTGCATATGAAGCGGTCATAGATTCCGGAATAAACCCAAAAAGTCTTCGGGGATCAAAAACTGGAGTGTACATTGGATCTTGTATTTCTGAATCAGAAAAAACATGGTTTTATGAAAAAGTTTCATCTGGTGGATTTGGAATAACTGGCTGCAGTCGGGCTATGATGGCTAATAGAATATCTTACTGTTTAGGACTCGAGGGACCTTCTTTTCTATTAGATACTGCATGTTCAAGTTCAATGTACGCGTTGGATAATGCCTTTAGTGCACTTCGAAATGGAGAAATAGATGCAGCAATAATTGGTGGCTCCAACTTACTTCTTCATCCATTTGTGACTCTTCAATTTGCTCGGTAAGCTTTTTGATTTTTTTAAAATTTTATTTCCTAACAAACTTTAAATTCCATAACATCATAGACTCGGCGTACTTGCTCCAGATGGGTTTTGTCGTCCATTCGATAAAAATGCAAGTGGCTATACCAGATCTGAAACTATCAATTGCCTATTTTTGCAACGAAAACGGGATGCAAAGCGTGTGTATGCTAGTGTTGTGTATTCAAAAACTAATTGTGATGGATATAAGCCAGAGGGTATAACGTATCCATCTGGGGTAGTCCAAGAAAAGTTGTTGGAAAATTTCTATAAAGAGATCGAACTTACCCCCTCAGATTTGGGCTATTTAGAGGCTCACAGTACTGGAACTGTAGTTGGTGATCCAGAAGAATGCAAAGCAATAGACAATGTAATTTGTAGCCAAAGACGCGAGCCTTTATTAATTGGATCTGTTAAATCAAATATTGGACATTCCGAAGCTGCATCAGGAATATGCTCACTTGTTAAAGCTTGCTTTGCATTTGAAACTGGCATGATTGCACCGAATATAAATTTTGCAGAAGTAAAACCAATTATTGCACCTCTAGCAGAAGGAAGATTGCTTGTTGTTAAAGATAAAACGCCTCTACCAAAGCCATACATCGGTATAAATTCTTTTGGATTTGGAGGTGCAAATGCACATGCACTTCTGAAAGGTTTTACCAAATCAAAGACAAATTTTGGGATTCCTGAGGATGATGTACCTCGACTTCTTACATGGGCCGGTAGGACAGAAGATGCAGTGCATGAAATTTTTAGCGACATAGAAAAAAGACCCCTAGATGCAGAATTTATCGGACTACTTCAAAATATTCAAGAGG