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5‘-TTTTT CTCGAG TCACACCACCACCACCACCACCTTAGA AATAAACCTATCATATATGTACC-3'

cloning into pET21a+ expression vector. w BmLspAf (NdeI). 5'-TTTTT CATATG AAAAAACTATGCTTGATTATTACACC-3'. w BmLspAr (XhoI). 5'-TTTTT CTCGAG CTTAGAAATAAACCTATCATATATGTACC-3'. Ec LspAf (NdeI). 5'-TTTTT CATATG AGTCAATCGATCTGTTCAACC-3'. Ec LspAr (XhoI).

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5‘-TTTTT CTCGAG TCACACCACCACCACCACCACCTTAGA AATAAACCTATCATATATGTACC-3'

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  1. cloning into pET21a+ expression vector wBmLspAf (NdeI) 5'-TTTTTCATATGAAAAAACTATGCTTGATTATTACACC-3' wBmLspAr (XhoI) 5'-TTTTTCTCGAGCTTAGAAATAAACCTATCATATATGTACC-3' EcLspAf (NdeI) 5'-TTTTTCATATGAGTCAATCGATCTGTTCAACC-3' EcLspAr (XhoI) 5'-TTTTTCTCGAGTTATTGTTTTTTCGCTCTAGAAGG-3' cloning into pGEX5.1 expression vector wBmLspAf (BamHI) 5'-TTTTTGGATCCTGAAAAAACTATGCTTGATTATTACACC-3' 5‘-TTTTTCTCGAGTCACACCACCACCACCACCACCTTAGA AATAAACCTATCATATATGTACC-3' wBmLspAr (XhoI)

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