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004H08

004H08. Carrot cot-1 DNA. Merge. Fig. S1 FISH analysis of BAC clone 004H08, which harbors the Cent-Dc tandem repeat, and carrot cot-1 DNA. Bar scale is 5 µm. Chr. 1. LG 1. Chr. 6. LG 3. Chr. 3. LG 8. Chr4. LG6. 0.0. 0.0. 103.3. 0.0. 18S-25S rDNA. 0.0. 0.2. 067N21. 068M03.

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004H08

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  1. 004H08 Carrot cot-1 DNA Merge Fig. S1FISH analysis of BAC clone 004H08, which harbors the Cent-Dc tandem repeat, and carrot cot-1 DNA. Bar scale is 5 µm

  2. Chr. 1 LG 1 Chr. 6 LG 3 Chr. 3 LG 8 Chr4 LG6 0.0 0.0 103.3 0.0 18S-25S rDNA 0.0 0.2 067N21 068M03 0.01 DCOR 71.8 34.0 GSSR-101 38.9 CRTISO 019A16 0.37 LCYB 57.5 0.56 025M09 0.83 31.4 032K14 0.66 GSSR-17 0.82 069N01 0.88 A9P11 238G06 0.0 110.3 139.6 Chr. 7 LG 5 Chr. 9 LG 7 0.0 0.0 208.6 112G15 0.02 113N07 0.34 SSR-W93 63.8 0.81 003E10 ZEP 97.5 0.98 004J17 Y2mark 121.5 Bac clone 154.2 Centromeric region 143.5 Fig. S2Relationship between pachytene chromosomes 1, 3, 4, 6, 7 and 9, and carrot (D. carota line B493)genetic linkage groups 1, 8, 6, 3, 5, and 7. For each chromosome, BAC clones are placed according to their relative distance from the end of the short arm. Dashed lines linking cytogenetic and genetic maps are drawn only between the map-anchored BAC clones and the corresponding genetic markers. Relative orientation between chromosome 4 and LG 6, chromosome 6 and LG3, and chromosome 9 and LG 7 is ambiguous

  3. 067N21 238G06 18S-25S rDNA 032K14 a b c 025M09 032K14 d Fig. S3 FISH mapping of carrot chromosome 3-specific BACs on the somatic chromosomes of D. crinitus suggested the occurrence of at least one translocation and one inversion between these two species. (a) Somatic chromosomes of D. crinitus probed with BACs 067N21 (green) and 032K14 (red). (b) The same preparation reprobed with BAC 238G06 (red, arrowed) and (c) with 18S-25S rDNA (green). BAC 238G06 does not co-localized on the same chromosome as the former BACs (as in carrot), and it is located on the long arm of the chromosome pair bearing the 18S-25S rDNA. (d) Partial metaphase of D. crinitus probed with 032K14 (red) and 025M09 (green), indicating the inverted order of these BACs compared to carrot. Bar scale=5 µm

  4. A8N14 A8N14 A6F01 A6F01 D. crinitus a D. pusillus b 003E10 112G15 004J17 D. crinitus D. crinitus c d Fig. S4 Examples of comparative FISH mapping of carrot chromosome-specific BACs in D. crinitus and D. pusillus (both 2n=22). (a-b) Two carrot chromosome 5-specific BACs (red and green signals) hybridized to two different chromosome pairs in both D. crinitus and D. pusillus. (c-d) FISH mapping of carrot chromosome 7-specific BACs in D. crinitus indicated that the order of these clones is the same as in carrot. (c) D. crinitus chromosomes probed with BACs 003E10 (green signal) and 004J17 (red signal). (d) The same metaphase probed with BAC 112G15 (red signal). Bar scale=5 µm

  5. 5S rDNA 064A04 124B06 5S rDNA 002B20 124B06 a b c Fig. S5FISH mapping of carrot chromosome 8-specific BACs on the somatic chromosomes of D. crinitus. (a) D. crinitus probed with 5S rDNA (green) and 064A04 (red). (b) The same preparation probed with 124B06 (red). (c) D. crinitus chromosomes probed with 5S rDNA (green), 002B20 (red) and 124B06 (green). Bar scale=5 µm

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