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Sea Urchin Vitelline Envelope Removal

Sea Urchin Vitelline Envelope Removal. Devin Morris Maurice Fernandez Milton Diaz. Question. Do the two different methods used in VE removal (Alpha-Amylase & DTT) yield the same data in 10% and 12% gels, or does one yield more accurate results than the others?.

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Sea Urchin Vitelline Envelope Removal

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  1. Sea Urchin Vitelline Envelope Removal Devin Morris Maurice Fernandez Milton Diaz

  2. Question Do the two different methods used in VE removal (Alpha-Amylase & DTT) yield the same data in 10% and 12% gels, or does one yield more accurate results than the others?

  3. Removal of Vetelline Envelope Vitelline Envelope - Outer membrane of female egg that plays a role in fertilization α-Amylase - Enzymes used to break up starches Dithiothreitol - DTT - Reduces disulfide bonds in proteins and enzymes. Manual - Manually remove the Vitelline Envelope

  4. Gels Measured 10% Acrylamide gel Concentration 12% Acrylamide gel Concentration

  5. Lanes - Adjusted brightness and contrast on lanes to make better measurements of bands - Measured each band length 10% Manual 12% Manual

  6. Linear

  7. Quadratic

  8. Cubic

  9. 10% Linear Model

  10. 10% Quadratic Model

  11. 10% Cubic Model

  12. 10% Non-Standard Model Y=ax^3+bx+c

  13. Linear

  14. Quadratic

  15. Cubic

  16. 12% Linear Model

  17. 12% Quadratic Model

  18. 12% Cubic Model

  19. 12% Non-Standard Model

  20. Conclusion - Based on our data analysis and calculations we concluded that the cubic model fits better for 10% and 12%.

  21. Alpha - amylise 10% Cubic vs Non-Satandard

  22. DTT 10% Non-standard vs Cubic

  23. 10% Manual Non-standard vs Cubic

  24. Alpha - amylise 12% Cubic vs Non-standard

  25. DTT 12% Non-standard vs Cubic

  26. 12% Manual Cubic vs Non-standard

  27. Conclusion Analysis of the confidence intervals produced by the cubic and non-standard models showed the non-standard model to work better for the 10% gel and the cubic to work better for the 12% gel. - In addition the DTT process of removal of the Vitelline envelope is better than the α-Amylise process for both the Non-standard and cubic models in both 10% and 12% gels.

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