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SLBP

Choe _ Fig S1. A. B. After IP ( a -FLAG). His-CTIF (365-598). Before IP. CTIF. CTIF. pcDNA3-FLAG. SLBP(128-270). SLBP(128-270). SLBP(1-127). SLBP(1-127). GST-SLBP(1-127). GST-SLBP(1-127). pCMV-Myc. GST- Trx. GST- Trx. SLBP. SLBP. SLBP. SLBP. GST. GST. kDa. 40.

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SLBP

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  1. Choe_Fig S1 A B After IP (a-FLAG) His-CTIF (365-598) Before IP CTIF CTIF pcDNA3-FLAG SLBP(128-270) SLBP(128-270) SLBP(1-127) SLBP(1-127) GST-SLBP(1-127) GST-SLBP(1-127) pCMV-Myc GST-Trx GST-Trx SLBP SLBP SLBP SLBP GST GST kDa 40 GST-SLBP (1-127) FLAG-CTIF GST-Trx Myc-SLBP 35 GST 25 Myc-SLBP(128-270) His-CTIF (365-598) Myc-SLBP(1-127) Input GST pull-down CBP80 b-actin Merge DAPI VFP C VN VC D SLBP(WT)-VN CTIF-VC E SLBP(W75A)-VN CTIF-VC

  2. Choe_Fig S2 A Control CTIF siRNA 10 20 30 40 10 20 30 40 Min past HU 0 0 HIST2H2AA mRNA SLBP(WT) SLBP(W75A) pcDNA3-FLAG Min past HU H3F3A mRNA FLAG-SLBP(WT or W75A) 95 3 69 9 49 6 33 0 100 HIST2H2AA mRNA / H3F3A mRNA (%) -actin 96 7 62 9 908 718 100 B 10 10 20 20 30 30 40 40 0 0 160 140 120 100 HIST2H2AA mRNA /H3F3A mRNA (%) 80 C 60 Control siRNA 40 20 0 hr 4hr 6hr 8hr 10hr 250 250 250 250 250 0 Min past HU 0 10 20 30 40 0 10 20 30 40 Control CTIF siRNA 2N 4N 2N 4N 2N 4N 2N 4N 2N 4N CTIF siRNA 0 hr 4hr 6hr 8hr 10hr 250 250 250 250 250 2N 4N 2N 4N 2N 4N 2N 4N 2N 4N D

  3. Choe_Fig S3 A B Control CTIF siRNA + + Control CTIF siRNA HU + + HU Uridylated HIST2H2AA mRNA CTIF H3F3A mRNA b-actin 26954 Uridylated HIST2H2AA mRNA / H3F3A mRNA (%) 100 11833 100

  4. Choe_Fig S4 A SLBP(WT) pcDNA3-FLAG Min past HU 10 20 30 40 10 20 30 40 0 0 FLAG-SLBP(WT) -actin B SLBP(WT) pcDNA3-FLAG Min past HU 10 20 30 40 10 20 30 40 0 0 HIST2H2AA mRNA H3F3A mRNA 89 5 67 2 43 2 34 2 100 HIST2H2AA mRNA / H3F3A mRNA (%) 90 3 36 6 725 508 100

  5. Choe_Fig S5 A eIF4E-BP1 pCMV-Myc + + HU Myc-eIF4E-BP1 -actin B C 120 120 100 100 80 80 Relative RLuc activity / RLuc mRNA (%) RLuc mRNA / SMG7 mRNA (%) 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-BP1 pCMV-Myc eIF4E-BP1 D E 120 120 100 100 HIST1H1C mRNA / H3F3A mRNA (%) HIST2H2AA mRNA / H3F3A mRNA (%) 80 80 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-BP1 pCMV-Myc eIF4E-BP1

  6. Choe_Fig S6 A B 160 140 120 100 eIF4E-T Dicistronic mRNA / SMG7 mRNA (%) pCMV-Myc 80 + + 60 HU 40 Myc-eIF4E-T 20 -actin 0 + + HU pCMV-Myc eIF4E-T C D 5 1.5 4 1 Relative FLuc activity / dicistronic mRNA Relative RLuc activity / dicistronic mRNA 3 2 0.5 1 0 0 + + + + HU HU pCMV-Myc eIF4E-T pCMV-Myc eIF4E-T E F 120 120 100 100 80 80 HIST1H1C mRNA / H3F3A mRNA (%) HIST2H2AA mRNA / H3F3A mRNA (%) 60 60 40 40 20 20 0 0 + + + + HU HU pCMV-Myc eIF4E-T pCMV-Myc eIF4E-T

  7. Choe_Fig S7 A B CBP80/20-dependent translation eIF4E-dependent translation Stop Stop eIF4GI/II eIF3 Cap eIF4E AUG SLIP1 CTIF Histone mRNA decay 80 60S eIF3 Genotoxic stress Cap AUG 20 40S 40S 20 3’-5’ decay CTIF SLBP SLBP Stop Stop SLBP eIF3 SLIP1 SLIP1 eIF4GI/II eIF3 AUG Cap eIF4E AUG 80 Cap 5’-3’ decay Dcp1/2

  8. Choe_Fig S8 FLAG-SLBP + Cross-link a-FLAG a-FLAG IP mIgG mIgG FLAG-SLBP CTIF CBP80 eIF3b eIF4GI eIF4E b-actin

  9. Choe_Fig S9 Myc-SLIP1 After IP (a-FLAG) Before IP + + RNase A SLBP(W75A) SLBP(W75A) SLBP(W75A) SLBP(W75A) SLBP(WT) SLBP(WT) SLBP(WT) SLBP(WT) pcDNA3-FLAG FLAG-SLBP CTIF Myc-SLIP1 eIF4GI eIF4E b-actin

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