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Geobacter

Investigating a potential mechanism of Mercury Uptake in Geobacter sulfurreducens. Geobacter. Geobacter sulfurreducens PCA chromosome. 1463922. 1468286. Heavy Metal Binding Domain containing protein. ABC Transporter Permease. CxxC motif. Graphic from NCBI database.

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Geobacter

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  1. Investigating a potential mechanism of Mercury Uptake in Geobactersulfurreducens Geobacter

  2. GeobactersulfurreducensPCA chromosome 1463922 1468286 Heavy Metal Binding Domain containing protein ABC Transporter Permease CxxC motif Graphic from NCBI database

  3. Sequence Alignments HMA domain GMTCxxC Signal peptide Periplasmic location

  4. GSU1338 and merP comparison Next Step:

  5. Experimental Approach • PCR • DNA extraction from gel • Enzyme Digestion & Ligation • Transformation • Harvest and sequence • Incubate cells until OD600 .4-.6 • Induce with IPTG to final concentration .4mM • Run HisLink Nickel Column

  6. Ladder Total 3 4 5 6 7 8 9 10 Induced 8/9 10 kda

  7. Next step: competition experiments • Binding assays • Use merP as + control • Have – control • Titrate protein into assay with Hg bound to something that we understand i.e. cysteine

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