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Nucleic Acids DHPLC Applications in Biomedicine

In The Name of Allah. Nucleic Acids DHPLC Applications in Biomedicine. Pooria Gill PhD of Nanobiotechnology pooriagill@yahoo.com. Nucleic Acids. DNA Linear Nature-made Genomic Cytoplasmic Man-made Oligo -primer Oligo -probe Q-DNA Circular Nature-made Mitochondric Organoplasmic

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Nucleic Acids DHPLC Applications in Biomedicine

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  1. In The Name of Allah Nucleic Acids DHPLCApplications in Biomedicine Pooria Gill PhD of Nanobiotechnology pooriagill@yahoo.com

  2. Nucleic Acids • DNA • Linear • Nature-made • Genomic • Cytoplasmic • Man-made • Oligo-primer • Oligo-probe • Q-DNA • Circular • Nature-made • Mitochondric • Organoplasmic • Man-made • Vectors • RNA • Nature-made • rRNA • mRNA • tRNA • ncRNA • tmRNA • Man-made • siRNA

  3. Applications • DHPLC Systems (e.g. WAVE Systems) can be used for a wide range of applications including: • Mutation Detection • Sizing • Oligonucleotide Purification and Analysis • Forensics • Microbial Analysis • RNA Isolation and Purification • Single Base Extension • Methylation

  4. With the WAVE System you can: • Scan fragments for both known and novel mutations/SNPs without extensive resequencing • Detect low-abundance mutations in heterogeneous samples • Enrich for low-abundance alleles • Fractionate complex mixtures of related fragments based on differences in sequence content • Perform targeted mutation/SNP scoring via primer extension assays • Analyze end-point products from allele-specific Probe/Primer-based Amplification Technologies

  5. Probe/Primer-based Amplification Technologies • Primer-based Amplifications: • Non-Isothermal Amplifications e.g. PCR-based Methods • Isothermal Amplifications/Non-PCR-based Amplifications: • NASBA • HDA • SDA • , and more than 45 N.A.I.A. Technologies • Probe-based Amplifications: • For example: Ligase Chain Reactions

  6. CARTRIDGE dependent Applications e.g. via WAVE Systems • DNA-99-3510 DNASep Cartridge 4.6 mm x 50 mm, for variety of DNA applications including Sizing and Mutation Detection, load capacity of 2µg • DNA-99-3710 DNASep HT Cartridge 6.5 mm x 37 mm, for variety of DNA application but especially for Rapid Mutation Detection, load capacity of 4µg • NUC-99-3550 OligoSep® Cartridge 4.6 mm x 50 mm; for Oligo Purification work only • NUC-99-3860 OligoSep Prep HC Cartridge 7.8 mm x 50 mm, for Oligo Purification, load capacity of 5000µg • RNA-99-3810 RNASep® Prep Cartridge 7.8 mm x 50 mm, for variety of RNA applications, load capacity of 50µg total RNA -OR- 1000ng of specific RNA

  7. Chromatograms and pedigree of patients 3 and 16. A. DHPLC analysis in normal control, patients 3 and 16. Both patients show abnormal DHPLC peak patterns with two peaks in comparison to normal control. B. Sequencing data in patient 3 and his family members. C. Sequencing data in patient 16 and his family members.

  8. Example of Oligo Analysis by DHPLC

  9. Thanks for Your AttentionsReference:

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