1 / 32

Antigenicity

Antigenicity. Requirements to be an immunogen Low mw antigens (haptens) Adjuvants Primary vs secondary responses. Concept of a Hapten. r/c = Kn-Kr. c = concentration of free antigen (hapten) K = K a = equilibrium constant r = hapten bound per unit Ab n = Valence of Ab. Polyclonal Ab.

hal
Download Presentation

Antigenicity

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Antigenicity • Requirements to be an immunogen • Low mw antigens (haptens) • Adjuvants • Primary vs secondary responses

  2. Concept of a Hapten

  3. r/c = Kn-Kr c = concentration of free antigen (hapten) K = Ka = equilibrium constant r = hapten bound per unit Ab n = Valence of Ab

  4. Polyclonal Ab

  5. 1/c = K(2) – K(1) 1/c = K0

  6. Factors which influence Ab affinity • Temp, pH, ionic strength • Polyclonal vs monoclonal • Ab class • Type of antigen

  7. Antibody Combining Site Location

  8. Figure 3-4

  9. Figure 3-1

  10. Evidence that Hv interacts with Ag 3 lines of evidence

  11. Affinity Labeling

  12. Comparison of Hv sequences of monoclonals binding the Same vs binding a different antigen epitope

  13. All interactions are non-covalent

  14. Affinity vs avidity – depends on valence

  15. IgM is the most avid of Ab classes

  16. Composition of immune ppts at different Ab/Ag ratios

  17. Gel Diffusion Analysis to determine relatedness of antigens

More Related