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Puget Sound EPA Benthos Grant: Comparison of Sampling Methods and Updated Taxa Attributes

Jo Wilhelm & Deb Lester, King County Leska Fore, Statistical Design Karen Adams, WA Department of Ecology Gretchen Hayslip, EPA Region 10. Puget Sound EPA Benthos Grant: Comparison of Sampling Methods and Updated Taxa Attributes.

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Puget Sound EPA Benthos Grant: Comparison of Sampling Methods and Updated Taxa Attributes

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  1. Jo Wilhelm & Deb Lester, King County Leska Fore, Statistical DesignKaren Adams, WA Department of EcologyGretchen Hayslip, EPA Region 10 Puget Sound EPA Benthos Grant: Comparison of Sampling Methods and Updated Taxa Attributes King County Water and Land Resources Science Seminar November 1, 2012

  2. Overview • Regional monitoring issues that initiated this project • Key Project Goals • Methods and Preliminary Results • Reconcile differences in sampling methods • Update taxa attributes • Next steps EPA Scientific Studies and Technical Investigation Assistance Program Support technical studies to guide and evaluate implementation of PSP’s Action Agenda

  3. Goal: Improved decision making to restore and protect streams

  4. Reconcile Differences in Sampling Methods • Ecology requires >=8ft2 samples for inclusion in State WQ Assessment • Reluctance to shift to 8ft2 - concern for orphaned data • Need for better understanding of data comparability or tool to allow data comparability

  5. Sample Collection Methods – 3ft2 vs. 8ft2

  6. Sampling Locations • 55 Sites • 9 Partners • Elevation 4-330 m • 0-93% Urban

  7. Results: Overall BIBI Score - 3 vs. 8 sq ft

  8. Overall BIBI Score: Residuals Mean = 1.2 p<0.05 Biologically meaningful?

  9. Individual BIBI Metrics

  10. Paired Sample Analysis Conclusions A little more analysis needed, but… No additional 2012 sampling No “cross-walk” required Data are comparable

  11. Strengthen Sensitivity of Taxa Attributes Update Using Published Literature Update with Existing Data

  12. Published Literature Updates

  13. Attribute Changes: 1998 vs. 2012

  14. Tolerant & Intolerant Taxa Testing • N = 784 sites (most recent) • Genus level or higher • >= 25 occurrences • 155 taxa tested 1.0 0.8 0.6 Cumulative % of Sites 0.4 0.2 Epeorus 0.0 0 20 40 60 80 100 % Urbanization in Watershed

  15. Example of an Intolerant Taxon 1.0 0.8 0.6 Cumulative % of Sites 0.4 Epeorus 0.2 0.0 0 20 40 60 80 100 % Urbanization in Watershed

  16. Example of a Tolerant Taxon 1.0 Erpobdellidae 0.8 0.6 Cumulative % of Sites 0.4 0.2 0.0 0 20 40 60 80 100 % Urbanization in Watershed

  17. Attribute Changes: 1998 vs. 2012

  18. BIBI Scores: Attributes Compared

  19. BIBI Metrics: Influence of Attributes * All mean residuals are significantly different than 0 (p<0.05)

  20. Taxa Attribute Conclusions • Significant changes to attribute lists, especially predator, long lived and tolerant/intolerant taxa • Many rare taxa dropped from tolerant and intolerant lists • No change to structure of B-IBI – all metrics highly correlated with % urbanization • Taxa attribute updates may require some recalibration

  21. Next Steps Finalize attributes Recalibrate BIBI and adjust scoring Reanalyze 3 vs. 8 with updated attributes Incorporate changes into PSSB Biological Condition Gradient process/Indicator refinement Ongoing collaboration

  22. Acknowledgements

  23. Deb Lester deborah.lester@kingcounty.govJo Wilhelmjo.wilhelm@kingcounty.gov www.pugetsoundstreambenthos.org

  24. Sample Processing

  25. Overall BIBI Score: Landcover

  26. Lab Methods WE ARE HERE

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