1 / 13

Green Fluorescent Protein

Green Fluorescent Protein. a B/MB senior seminar brought to you by Colm O’Carroll. This presentation will cover . The structural aspects of GFP which make fluorescence possible The advantages of using GFP and GFP mutants over other fluorescent markers

halil
Download Presentation

Green Fluorescent Protein

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Green Fluorescent Protein a B/MB senior seminar brought to you by Colm O’Carroll

  2. This presentation will cover • The structural aspects of GFP which make fluorescence possible • The advantages of using GFP and GFP mutants over other fluorescent markers • The use of GFP to monitor viral movement in plants

  3. The Green Fluorescent Protein

  4. GFP’s unique structure • Composed of 238 amino acids • “Paint in a can” • Each monomer composed of a central -helix surrounded by an eleven stranded cylinder of anti-parallel -sheets • Cylinder has a diameter of about 30A and is about 40A long • Fluorophore located on central helix

  5. The Active Site

  6. The Fluoropore Active Site • Ser65-Tyr66-Gly67 • Deprotonated phenolate of Tyr66 is cause of fluorescence • Forster Cycle (1949-Theodor Forster) • Proton transfer to His148

  7. Fluorophore formation • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo • Renaturation most likely by a parallel pathway • Oxidation of Fluoropore (2-4 hours) • Two step process

More Related