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The Frequency Dependence of Osmo -Adaptation in Saccharomyces cerevisiae

The Frequency Dependence of Osmo -Adaptation in Saccharomyces cerevisiae. Alexander van Oudenaarden Lab 20.309 Final Presentation Mashaal Sohail Emily Suter. Motivation. Cells respond to stimuli using complicated systems of biochemical reactions

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The Frequency Dependence of Osmo -Adaptation in Saccharomyces cerevisiae

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  1. The Frequency Dependence of Osmo-Adaptation in Saccharomycescerevisiae Alexander van Oudenaarden Lab 20.309 Final Presentation Mashaal Sohail Emily Suter

  2. Motivation • Cells respond to stimuli using complicated systems of biochemical reactions • Systems may comprise of hundreds of reactions • Interest in determining dominant processes that dictate system dynamics

  3. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response

  4. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response Hog1 cell nucleus

  5. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response high osmolarity solution

  6. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response

  7. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response

  8. Hog1 MAPK Pathway High-Osmolarity Glycerol (HOG) Mitogen-Activated Protein Kinase (MAPK) pathway in budding yeast Saccharomyces cerevisiae • Core pathway of the hyperosmotic shock response

  9. Experimental Setup

  10. Experimental Setup

  11. Experimental Setup

  12. Experimental Setup

  13. Fourier analysis approximates output signal as sin curve

  14. Linear time invariant model predicts system response to input signal Hog1 Response to Single NaCl Step

  15. Pulsed-input analysis corresponds to biological network

  16. Hog1-dependent feedback loop plays major role in rapid response Hog1 Response to Single NaCl Step

  17. Gene expression facilitates response to osmotic shocks on longer time scales Wild Type Inhibited Hog1 Expression Response R(t) time [min]

  18. Significance/Conclusions • Showed that Hog1 MAPK pathway is governed by two different response time scales • Demonstrated use of frequency-response analysis on cellular networks • Utilized engineering principles to predict the response of dynamic stimuli

  19. Significance/Conclusions • Showed that Hog1 MAPK pathway is governed by two different response time scales • Demonstrated use of frequency-response analysis on cellular networks • Utilized engineering principles to predict the response of dynamic stimuli Thank you

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