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HBsAg+ candidate blood donors

HBsAg+ candidate blood donors. HBV genotypes Number Origin(s). A1 43 S. Africa. A2 72 Poland. B 126 Hong Kong Malaysia Taiwan Thailand. C 90 Hong Kong Malaysia Taiwan Thailand. D 465 Egypt Iran Poland S. Africa Turkey Tunisia. E 184 Guinea.

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HBsAg+ candidate blood donors

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  1. HBsAg+ candidate blood donors HBV genotypes Number Origin(s) A1 43 S. Africa A2 72 Poland B 126 Hong Kong Malaysia Taiwan Thailand C 90 Hong Kong Malaysia Taiwan Thailand D 465 Egypt Iran Poland S. Africa Turkey Tunisia E 184 Guinea HBsAg quantification: Architect HBsAg, Abbott Lab. (0.05 IU/ml or 0.28 ng/ml) HBV DNA quantification: in-house real-time QPCR assay (<20 IU/ml)

  2. Relationship between HBsAg and HBV DNA levels A1 A2 B 5 6 5 5 4 4 4 HBsAg (log IU/ml) HBsAg (log IU/ml) 3 HBsAg (log IU/ml) 3 3 2 2 2 R2=0.08 1 1 R2=0.02 1 R2=0.39 2 4 6 8 10 2 4 6 8 10 2 4 6 8 10 12 HBV DNA (log IU/ml) HBV DNA (log IU/ml) HBV DNA (log IU/ml) n=43 n=126 n=72 C D E 6 6 6 5 5 5 4 4 4 HBsAg (log IU/ml) HBsAg (log IU/ml) HBsAg (log IU/ml) 3 3 3 2 2 2 R2=0.31 R2=0.17 1 1 1 R2=0.22 2 4 6 8 10 2 4 6 8 10 2 4 6 8 10 HBV DNA (log IU/ml) HBV DNA (log IU/ml) HBV DNA (log IU/ml) n=465 n=184 n=90

  3. A1 A2 60 30 40 20 20 10 0 0 <1 1 1 >1 <1 1 >1 B C 20 25 10 10 0 0 <1 1 >1 <1 1 >1 E 30 20 10 0 <1 1 >1 HBsAg / HBV DNA ratio n=43 n=72 Frequency (%) n=126 n=90 D 30 n=465 n=184 20 10 0 <1 1 >1 Ratio

  4. Chinese - genotype B Chinese - genotype C 20 30 20 10 10 0 0 <1 1 >1 <1 1 >1 Malay - genotype B Malay - genotype C 30 30 20 20 10 10 0 0 <1 1 >1 <1 1 >1 Thai - genotype B Insufficient data Thai - genotype C 30 20 10 0 <1 1 >1 HBsAg / HBV DNA ratio according to ethnic groups

  5. Conclusions • HBsAg range is between 102 and 106 IU/ml for genotypes A, C and E. Significantly lower values are seen in genotypes B and D. • A1, B and D have lower median DNA than genotypes A2, C and E • No correlation between plasma levels of HBsAg and viral load irrespective of genotype • Very low HBsAg (3.7%) or DNA (6.2%) levels are observed in genotype D • Within genotype B infection HBsAg and DNA levels are significantly higher in Chinese than Malays

  6. Acknowledgements B Meldal P Garmiri D Belkhiri Dept Haematology University of Cambridge, UK K Lin, HKRCBTS, Hong Kong T Sakul, NRCBTS, Bangkok, Thailand M Vermeulen, SANBTS, Durban, SA E Brojer, NIHTM, Warsaw, Poland A Loua, CNTS, Conakry, Guinea H Bon, NBTS, Kuala Lumpur, Malaysia K Boukef, NBTS, Tunis, Tunisia O Arslan, Ankara, Turkey H Zadeh, IBTO, Teheran, Iran M El Ekiaby, Cairo, Egypt B Foglieni Dept of Transfusion Medicine and Hematology, Lecco, Italy Supported by ISBT Foundation NHSB&T, England Novartis

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