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pAMPK

Metformin (t). 30 ’. 5 ’. 15 ’. pAMPK. AMPK. β -act. Metformin (t). 30 ’. 60’. 24h. 5 ’. 15 ’. pNF-  B. NF-  B. β -act. GAPDH. Supplementary Fig. 1. A. Metformin (mM). Ctrl. 1. 10. 50. pAMPK. AMPK. β -act. B. Ctrl. C. Ctrl. ). %. (. n. o. i. t. a. l. u. p.

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pAMPK

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  1. Metformin (t) 30’ 5’ 15’ pAMPK AMPK β-act Metformin (t) 30’ 60’ 24h 5’ 15’ pNF-B NF-B β-act GAPDH Supplementary Fig. 1 A Metformin (mM) Ctrl 1 10 50 pAMPK AMPK β-act B Ctrl C Ctrl

  2. ) % ( n o i t a l u p o P Metf 10mM NT 6h 24h 48h Cyclin D1 CDK4 Vinculin Cyclin D1 CDK4 Metf 10mM Relative Expression NT 6h 24h 48h Pro-Caspase 3 - 6 24 48 - 6 24 48h Metf β-act Supplementary Fig. 2 B A 1 2 0 1 0 0 Dead Apop 8 0 MTT (OD540) G2/M 6 0 S 4 0 G1/G0 2 0 0 NT 6h 24h 48h Metf 10mM C p21 Cyclin D1 Relative mRNA levels Relative mRNA levels - + + - + + Metf Metf [10mM] [10mM] 6h 24h 24h 6h * * * D E N E C R O S I S Population (%) L A T E A P O P T O S I S E A R L Y A P O P T O S I S V I A B L E 1 0 0 S 8 0 L L E C C 6 0 % 4 0 2 0 0 NT 0.1 1 5 10 20 50 100 25mM 5-FU Metf (mM)

  3. Supplementary Fig. 3

  4. Supplementary Fig. 4 E Internal Ctrl VEGF-C CCL-2 MCF-7 HUVEC

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