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Le Zhang Zhongyan Shan Weiping Teng

Effect of Maternal Excessive Iodine Intake on Neurodevelopment and Cognitive Function in Rat Offspring. Le Zhang Zhongyan Shan Weiping Teng Department of Endocrinology and Metabolism, First Affiliated Hospital of China Medical University Shenyang, China. Background.

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Le Zhang Zhongyan Shan Weiping Teng

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  1. Effect of Maternal Excessive Iodine Intake on Neurodevelopment and Cognitive Function in Rat Offspring Le ZhangZhongyan Shan Weiping Teng Department of Endocrinology and Metabolism, First Affiliated Hospital of China Medical University Shenyang, China

  2. Background • Iodine is essential for the synthesis of thyroid hormone, especially during pregnancy • Inadequate iodine intake during gestation results in thyroid hormone deficiency and permanent changes in the neurological and cognitive functions of offspring BMC Neuroscience 2010, 11:50 BMC Neuroscience 2009, 10:149

  3. < 100 Thyroid diseases 100~200 >200 Urinary iodine Level (μg/L) Background • ss • There have been few studies regarding maternal mildly excessive iodine intake and associatedchanges in the neurodevelopment of offspring Thyroid 2001,11(5):457 N Engl J Med 2006, 354(26):2783–2793 Neurotoxicology 2005, 26(3):417–426

  4. Background Our previous study Excessive iodine intake could increase the risk of subclinical hypothyroidism in offspring Chin Med J (Engl) 2004, 117(10):1518-1522

  5. Background Our previous study Maternal overt and subclinical hypothyroidism affect the expression of BDNF Thyroid 2010, 20(8):909–915

  6. NSP-A NSP-A NSP-A NSP-A C-fosc-jun C-fosc-jun C-fosc-jun C-fosc-jun BDNF BDNF BDNF BDNF Background G17 PN7 PN45 Neurotoxicology 2012, 33(4):842-852  Neurotoxicology 2005, 26(3):417-426 Neurotoxicol Teratol 2011, 33(4):464-472

  7. Objective • Whether higher-than-normal iodine intake from before pregnancy until breastfeeding affects the postnatal neurodevelopment and cognitive function

  8. Materials AND Methods SPF female Wistar rats 80-120 g (n = 60) low iodine (n=20) (LI, deionized water) normal iodine (n=20) (NI, 140 μg /L KIO3) 3-fold iodine (n=20) (3HI, 480 μg /L KIO3) Mother Pup pre-pregnancy G17 PN7 PN45

  9. Thyorid hormone C-fos C-jun BDNF NSP-A Spatial memory Iodine content (urine, thyroid) Morris Water Maze Test Western blot Immunohisto- chemistry Arseniccerium catalyticSpec- trophotometry Chemilumines- Centimmuno- assay Materials AND Methods Mother Pup

  10. Results Urinary and thyroidal iodine content *P < 0.05 compared with normal iodine control group

  11. Results Maternal thyroid hormone *P < 0.05 compared with normal iodine control group

  12. Results Pup thyroid hormone *P < 0.05 compared with normal iodine control group

  13. Results Effect of 3HI on the protein expressions of c–Fos and c-Jun in the CA1 area of hippocampus *P < 0.05 compared with normal iodine control group

  14. Results Effect of 3HI on BDNF and NSP-A expression in the hippocampus # P < 0.01 compared with the NI group on the same day;* P < 0.05compared with the NI group on the same day

  15. Results Morris water maze (MWM) test # P < 0.01 compared with the NI group on the same day;* P < 0.05compared with the NI group on the same day

  16. Conclusion • The offspring in the 3HI group may have a mildly impaired learning capacity, which could be associated with a decrease in BDNF and an increase in NSP-A levels • The careful control of maternal iodine intake level is important to prevent neurodevelopmental defects in offspring

  17. Thank You!

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