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DigiBio

www.digibio.com. Centro Congressi Cavour Roma, 14 dicembre 2002. DigiBio. Biologia Digitale: il nesso tra memoria dell’acqua, pratiche alternative e medicina del futuro Jacques Benveniste, MD, Directeur de Recherche Didier Guillonnet, MS Informatique. v 1.4 US.

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DigiBio

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  1. www.digibio.com. Centro Congressi Cavour Roma, 14 dicembre 2002 DigiBio Biologia Digitale: il nesso tra memoria dell’acqua, pratiche alternative e medicina del futuro Jacques Benveniste, MD, Directeur de Recherche Didier Guillonnet, MS Informatique v 1.4 US

  2. Professor Benveniste, why is it so difficult for you to be accepted by the system? Drawing by JP Petit in "H2O" Science Innovante publisher

  3. Generalities • G.1b History The high dilution story (dubbed the "Memory of Water") 1984 Basophil degranulation triggered by high dilution of serum anti-IgE. 1988 Publication in Nature, followed by an ‘‘inquiry’’. 1991 Erasing of high dilution activities by an oscillating magnetic field. (collaboration with a CNRS team) 1992 Electronic transfer (via an amplifier) of biological information to a tube of water. 1995 Digitization: recording then replay of the biological message using a computer. 1998 Activation by agitation of a solution at very low concentration (down to 10-14M)

  4. Technologies T.1.2 - Biological Sensitive System -Langendorff

  5. EFFECT OF HOMEOPATHIC GRANULES (ARNICA montana) ON ISOLATED GUINEA-PIG HEARTS (% increase in coronary flow) GranulesMean + 1 SD (n) Arnica montana 15CH 16.0+ 1.2 (4)* Arnica montana 30CH 13.7+ 8.7 (3)* Inert 3.6 + 3.0 (4) Digitally-Recorded Granules (d) d-Arnica montana 15CH 19.4+ 3.3 (5)** d-Arnica montana 30CH 14.4+ 1.4 (3)** d-Inert 2.9+ 0.8 (3) Arnica montana MT (1/106) 29.9+10.6 (3) * p < 0.05 compared with inert granules ** p < 0.05 compared with d-inert granulesc DigiBio 1999

  6. BIOLOGICAL ACTIVITY IN HOMEOPATHIC GRANULES (Arnica montana) (Guinea-pig isolated heart - % var. in coronary flow) EthylicumArnica mont. Open/ Date of Date 15CH 15CH Blind purchase 18.9.98 4.8 13.8 Open June 98 21.9.98 5.4 13.5 Open June 98 24.9.98 5.4 19.1 Blind June 98 25.9.98 4.8 18.4 Blind Sept 98 30.9.98 5.6 2.9 25.0 22.9 Blind Sept 98 1.10.98 3.1 9.3 18.2 15.6 21.2 Blind Sept 98 5.10.98 4.0 20.0 Blind Sept 98

  7. EFFECTS OF HOMEOPATHIC GRANULES (ARNICA MONTANA) ON SKIN GUINEA-PIG (diameters of blue dots in mm) Mean + 1 SD (n) Arnica montana 15CH7,4,7,7,12,8 7.5 + 2.6 (6)* Arnica montana 30CH 16,12,18,20,17 16.6 + 2.9 (5)* Inert 2, 0, 0, 1, 0 0.6 + 0.8 (5) ____________________________________________________ Statistically significant diff. (Student’s t-test for unpaired variates): * p < 0.05 compared with inert granules

  8. Technologies T.1.5 - Biological Sensitive System -Skin Test Results in pixels x 10 3 1 ACh -12 M vortexed in saline (68.2) 7 ACh signal in water (1,294) 2 Same vortexed in 5 % glucose (59.5) 8 Same at low power (435) 3 Same vortexed in water (1,949) 9 Same as 6 (987) 4 Same mixed in water (44) 10 Acetate+Choline as in 3 (25) 5 Atropine + 3 (71) 11 ACh 1 µg (1,154) 6 Water + 3 (609) 12 Atropine + ACh 1 µg (36)

  9. THE ESSENTIAL ROLE OF WATER IN THE TRANSMISSION OF THE MOLECULAR SIGNAL * EFFECT OF VIOLENT AGITATION

  10. Technologies T.1.2.2 - Biological Sensitive System - Skin Test EFFECT ON GUINEA-PIG SKIN OF Vortexed vs Mixed ACETYLCHOLINE (1pM) or ACETATE-CHOLINE (1pM) Diam. of blue dots in mm ; mean + 1 SD (n) ACh 1pM (mixed) 1.6 +0.8 (11) ACh 1pM (vortexed) 12.9 +4.9 (17)* AC 1pM (vortexed) 1.3 +0.8 (9) AC 1pM (mixed) 1.4 +0.5 (7) ACh 1µM18.3+ 4.7 (11) * p < 0.05 compared with ACh 1pM mix. and AC 1pM mix. or vor. 21/10/98

  11. Technologies T.1.5 - Biological Sensitive System -Skin Test Results in pixels x 10 3 1 ACh -12 M vortexed in saline (68.2) 7 ACh signal in water (1,294) 2 Same vortexed in 5 % glucose (59.5) 8 Same at low power (435) 3 Same vortexed in water (1,949) 9 Same as 6 (987) 4 Same mixed in water (44) 10 Acetate+Choline as in 3 (25) 5 Atropine + 3 (71) 11 ACh 1 µg (1,154) 6 Water + 3 (609) 12 Atropine + ACh 1 µg (36)

  12. Skin Testing in the G-P: Effect of Dilution and Digital Signals(Comments on the June 5, 1998 Experiment) The experiment presented here shows that a specific biological activity can be obtained by “informing” water. It sums up a body of research which demonstrates: 1) the existence of biologically-specific signals, in the form of electromagnetic waves; 2) the role of water in the transmission and “memorization” of these signals. The experimental procedure has two aspects: 1) the activation, by simple mechanical agitation, of a molecular solution at a normally inactive concentration (1 x 10-12 M); this agitation reveals an activity in all ways comparable to that obtained with a typical active concentration (1 x 10-6 M); 2) using an electromagnetic coil, transmission to “naive” water of a specific electromagnetic signal pre-recorded on a computer.

  13. Technologies T.1.2 - Biological Sensitive System -Langendorff WATER IS ESSENTIAL IN TRANSFERRING THE MOLECULAR SIGNAL % increase in coronary flow mean + 1 SD (n) Signal recorded from: ACh 1 µM in 100% ethanol, VORTEXED 4.3 ± 1.8 (3) in 80% ethanol / 20% water, MIXED 8.0 ± 1.0 (3) in 80% ethanol / 20% water, VORTEXED 22.7 ± 3.5 (3) * * p < 0.05 compared with 100% ethanol

  14. Technologies T.3 - Biological Sensitive Systems -Operating modes MIMICKING THE SPECIFIC EFFECTS OF MOLECULES WITH COMPUTER-RECORDED SIGNALS (Low e.m. frequencies)

  15. Technologies T.5a Signal recording External EM field shielding container Emitter Receiver Signal modified by the sample White noise Electromagnetic coils Tube containing the substance to be recorded

  16. Technologies T.1.2.2 - Biological Sensitive System -Langendorff EFFECTS ON ISOLATED GUINEA-PIG HEARTS OF THE DIGITALLY-RECORDED (d) CAFFEINE % DECREASE in coronary flow. Mean + 1 SD (n) d-Caffeine 15.7 ± 4.9 (13) Caffeine (1µM): 22.3 ± 2.3 (3) d-Water (water recorded as control)4.6 ± 1.4 (6) 14/10/98

  17. The biological effect is similar whether it’s from a physical molecule or its signal obtained by HD

  18. Technologies T.1.2.2 - Biological Sensitive System -Langendorff EFFECTS ON ISOLATED GUINEA-PIG HEARTS OF THE DIGITALLY-RECORDED (d) IONOPHORE (1pM) (Vortexed vs Mixed) % increase in coronary flow mean + 1 SD (n) Iono 1pM 3.6, 5.1, 5.1, 4.6 4.6 ± 0.3 (4) (mixed) Iono 1pM 13.0, 42.9, 37.5, 11.1, 14.8 23.8 ± 6.7(5) (vortexed) Iono 1 µM39.6, 40.0, 50.043.2 ± 3.4 (3)

  19. Technologies T.1.6.4 Blood coagulation results Interaction of a person (whole body? Hands? Mind?) with the heparin signal as shown by «Sandwich» experiments 1) Ca2+-containing water is exposed to the heparin (or water as control) signal. 2) The exposed water-Ca2+ tubes are mixed with plasma and distributed in 96-well plates, first by an operator (JA), then by a second operator (SKL), then again by JA.

  20. Automatic Bio Analyser

  21. EFFECT OF HEPARIN SIGNAL Blind - January 20, 2000

  22. Technologies T.1.6.7 Blood coagulation results Interaction of a person with the heparin signal: Results and Discussion When SLK processed the tubes that were first found active in the hands of JA, the activity disappeared, most often definitively. Subsequent expts showed that this negative influence could be exerted from up to 1 meter distance (not shown). We have located another person with similar activity. At this time (Oct. 2000), we do not know the frequency of these « erasers » in the general population. Nor do we know whether or not there exists a link between this effect and other documented « paranormal » abilities. This method provides a pharmacological tool to routinely investigate such phenomena.

  23. Technologies T.1.6.6 Blood coagulation results « Sandwich » experiments * results (O.D. at 40 min) are means of duplicates

  24. Coherent domains in Water G. Preparata & E. Del Giudice

  25. Theoretical Considerations P.1 Molecular signalling The Current Theory:"structural matching" The Proposed Theory:"electromagnetic signals" The 3D structure of the ligand matches that of the receptor. Physical proximity induces receptor conformational changes which in turn triggers the cascade of events prompting cell function. Proximity favors resonance of ligand-receptor specific EM signals. Resonance serves as an amplifying mechanism for conformational changes which occur at all steps of the cascade inducing cell function.

  26. Conclusion (2) (Homeopathy) These advances permit to envision: - the implementation of a remote digital quality control aimed at insuring the actual presence of therapeutic activity in homeopathic products. - the future manufacturing of purely digital homeopathic products.

  27. Conclusion (General) • We can trigger specific biological effects with low frequency electromagnetic signals. • These biological signals are carried/transmitted by water. • They seem to support homeopathic activity. • They are sensitive to the influence of some individuals. • These influences may account for the difficulty in consistently replicating experimental systems, more than intrinsic variability of the latter.

  28. More Conclusions and a Question The experiments presented here show unequivocally that the mechanisms instrumental in homeopathy do belong to the realm of contemporary physics and biology. Therefore they can be investigated by usual scientific procedures. QUESTION: How can we explain that, homeopathic practices being under world-wide attacks (mainly based on the lack of scientific grounds) from « mainstream » science, no efforts are made by the industry and the practitioners to use a small percentage of their income to support fundamental research in homeopathy? TENTATIVE ANSWER: a suicidal attitude?

  29. Please check this reference Activation of human neutrophils by electonically transmitted phorbol-myristate acetate Y. Thomas, M. Schiff, L Belkadi, P. Jurgens, L. Kahhak, J. Benveniste. Medical Hypotheses (2000) 54(1), 33-39 Reprints at <yolene@cybercable.fr> or <jbenveniste@digibio.fr>

  30. A complementary Team • Jacques Benveniste • Exploration of new paths and deepening of the discovery • Also in charge of communication and external affairs • MD, Director of Research at INSERM - (National Medical Research Inst.) • Famous for the discovery of PAF (Platelet-Activating Factor) • Didier Guillonnet • Rationalization of protocols, Development of the automate, Signal processing • Also administration, management, legal affairs and intellectual property • MS Computer Science (Ecole Centrale de Paris) • Jamal Aissa (Ph.D), Larbi Kahhak (Ph.D), Soo K. Lim (MD, Ph.D) • Research Engineers in biology who have worked with J. Benveniste for many years, performing various experiments such as “isolated guinea pig heart” and “cell culture”… • Business angels experienced in company creation from seed to stock market • A patent lawyer widely recognized for his expertise in cutting edge technologies

  31. Contacts Jacques Benvenistejbenveniste@digibio.com Didier Guillonnetdguillonnet@digibio.com DigiBio S.A. 34 av. des Champs Elysées 75008 Paris - France Digital Biology Research Laboratory 32 rue des Carnets, 92140 Clamart - France Tel. +33 (0)1 46 01 58 40  Fax +33 (0)1 46 31 02 77 http://www.digibio.com

  32. MOTTO Los homeopatas unidos (y haciendo investigaciones) jamas seran vincidos. Pero no son unidos, hacen poca investigacion y cuando lo hacen, publican en periodicos noncientificos o confidenciales.

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