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UV-Vis Spectroscopy

UV-Vis Spectroscopy. http://www.cem.msu.edu/~reusch/VirtualText/Spectrpy/UV-Vis/spectrum.htm. UV-Vis Spectroscopy HPLC: Peak Area vs Detector setting. Excedrin ES 250 mg aspirin; 250 mg acetaminophen; 65 mg caffeine. Detector set at 240 nm. Area % Aspirin 19.5%

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UV-Vis Spectroscopy

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  1. UV-Vis Spectroscopy http://www.cem.msu.edu/~reusch/VirtualText/Spectrpy/UV-Vis/spectrum.htm

  2. UV-Vis Spectroscopy HPLC: Peak Area vs Detector setting Excedrin ES 250 mg aspirin; 250 mg acetaminophen; 65 mg caffeine Detector set at 240 nm Area % Aspirin 19.5% Acetaminophen 50.0% Caffeine 20.5% Detector set at 254 nm Area % Aspirin 7.3% Acetaminophen 81.9% Caffeine 10.8% Detector set at 280 nm Area % Aspirin 24.8% Acetaminophen 39.3% Caffeine 35.9%

  3. UV-Vis Spectroscopy: lmax 240 nm Acetaminophen (1.6 x 10-3 g/L) lmax = 248 nm, Abs. = 0.61 254 nm 280 nm Aspirin (1.6 x 10-3 g/L) lmax = 296 nm, Abs. = 0.18 Caffeine (1.6 x 10-3 g/L) lmax = 272 nm, Abs. = 0.34

  4. UV-Vis Spectroscopy: lmax and molar absorbtivity (e) Acetaminophen (1.6 x 10-3 g/L) lmax = 248 nm, Abs. = 0.61 Caffeine (1.6 x 10-3 g/L) lmax = 272 nm, Abs. = 0.34

  5. UV-Vis Spectroscopy: Chromophores http://www.cem.msu.edu/~reusch/VirtualText/Spectrpy /UV-Vis/spectrum.htm

  6. UV-Vis: Chromophores http://www.cem.msu.edu/~reusch/VirtualText/Spectrpy/UV-Vis/spectrum.htm

  7. UV-Vis Spectroscopy: Chromophores Table 7.3, p 362 Intro to Spectroscopy, 3rd ed., Pavia et al.

  8. UV-Vis Spectroscopy: Solvents p 358-9 Intro to Spectroscopy, 3rd ed., Pavia et al.

  9. UV-Vis Spectroscopy: CONJUGATION p 363 Intro to Spectroscopy, 3rd ed., Pavia et al. http://www.cem.msu.edu/~reusch/VirtualText/ Spectrpy/UV-Vis/spectrum.htm

  10. UV-Vis Spectroscopy: Aromatic Compounds p 375-76 Intro to Spectroscopy, 3rd ed., Pavia et al.

  11. UV-Vis: Aromatics p 379 Intro to Spectroscopy, 3rd ed., Pavia et al.

  12. UV-Vis: Acid/Base p 377 Intro to Spectroscopy, 3rd ed., Pavia et al.

  13. UV-Vis: Acid/Base Terminology for Absorption Shifts

  14. UV-Vis: Acid/Base

  15. UV-Vis: Empirical Rules p 380 Intro to Spectroscopy, 3rd ed., Pavia et al.

  16. UV-Vis: Empirical Rules p 380 Intro to Spectroscopy, 3rd ed., Pavia et al.

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