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CYP93E2

Supplementary Table 2 Estimated product yields of bAS/CPR/CYP716A12/CYP72A68v2 - and bAS/CPR/CYP93E2/CYP72A61v2 -expressing yeast.

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CYP93E2

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  1. tables and figures

  2. Supplementary Table 2 Estimated product yields of bAS/CPR/CYP716A12/CYP72A68v2- and bAS/CPR/CYP93E2/CYP72A61v2-expressing yeast Estimation was performed based on peak areas from the mass chromatograms shown in Figs. 2 and 3, by comparing the areas of internal standard and authentic compounds with known concentrations. tables and figures

  3. bAS CYP93E2 CYP72A61 UGT73K1 Supplementary Fig. S1 Expression profiles of P450 genes potentially involved in soyasapogenol B biosynthesis. Co-expression analysis was performed using the gene co-expression tool for Medicago truncatula (http://mtgea.noble.org/v2/correlation_search_form.php). Expression profiles for bAS, CYP93E2, CYP72A61, and UGT73K1 are shown. Corresponding probe IDs and correlation values are indicated in Table 1.

  4. bAS CYP716A12 CYP72A68 UGT73F3 Supplementary Fig. S2 Expression profiles of P450 genes potentially involved in gypsogenic acid biosynthesis. Co-expression analysis was performed using the gene co-expression tool for Medicago truncatula (http://mtgea.noble.org/v2/correlation_search_form.php). Expressions of bAS, CYP716A12, CYP72A68,and UGT73F3 are shown. Corresponding probe IDs are indicated in Table 2.

  5. Peak 4 in Fig. 3A Authentic erythrodiol (4) Supplementary Fig. S4 Mass spectrum of product resulting from transgenic yeast co-expressing bAS, CPR, CYP716A12, and CYP72A68. The mass spectrum of peak 4 shown in Fig. 3A matches up with that of authentic erythrodiol (4). Peak 2 inFig. 2A Authentic 24-OH-β-amyrin (2) Supplementary Fig. S3 Mass spectrum of product resulting from transgenic yeast co-expressing bAS, CPR, CYP93E2, and CYP72A61. The mass spectrum of peak 2 shown in Fig. 2A matches up with that of authentic 24-OH-β-amyrin (2). tables and figures

  6. Peak 4 in Fig. 4A Authentic erythrodiol (4) Peak 5 in Fig. 4A Authentic oleanolic acid (5) Peak 2 in Fig. 4A Authentic 24-OH-β-amyrin (2) Supplementary Fig. S5 Mass spectra of products resulting from transgenic yeast co-expressing bAS, CPR, CYP93E2, and CYP716A12. The mass spectra of peaks 2, 4, and 5 shown in Fig. 4A match up with those of authentic 24-OH-β-amyrin (2), erythrodiol (4), and oleanolic acid (5), respectively. tables and figures

  7. 26 18 19 20 21 22 23 24 25 bAS/CPR/CYP716A12/CYP72A63 A 14 15 16 17 18 5 4 Relative ion intensity (%) B bAS/CPR/CYP716A12 Retention time (min) Supplementary Fig. S6 In vivo production of queretaroic acid and other β-amyrin derivatives in transgenic yeast co-expressing bAS, CPR, CYP716A12, and CYP72A63. (A) and (B) show GC-MS analysis (TIC) of ethyl acetate extracts (using DB-1 MS column) from yeast cultures. (A) The yeast strains were engineered to express bAS, CPR, CYP716A12, and CYP72A63; (B) bAS, CPR, and CYP716A12 as controls. (C) The structures were deduced from mass spectra because authentic standards were not available. Queretaroic acid was identified by comparison with bibliographic data (Burnouf-Radosevich et al. 1985). tables and figures

  8. C Peak 4 in (A) Authentic erythrodiol (4) Peak 5 in (A) Authentic oleanolic acid (5) Peak 14 in (A) Authentic 30-OH-β-amyrin Peak 15 in (A) Peak 16 in (A) Peak 17 in (A) Queretaroic acid Peak 18 in (A) Supplementary Fig. S6 (Cont.) tables and figures

  9. β-Amyrin (1) bAS/CPR/CYP93E2/CYP72A63 A 2 14 20 21 15 19 22 Relative ion intensity (%) B bAS/CPR/CYP93E2 17 18 19 20 21 22 23 24 25 26 27 28 Retention time (min) Supplementary Fig. S7 In vivo production of rare triterpenoids in transgenic yeast co-expressing bAS, CPR, CYP93E2, and CYP72A63. (A) and (B) show GC-MS analysis (TIC) of ethyl acetate extracts (using DB-1 MS column) from yeast cultures. (A) The yeast strains were engineered to express bAS, CPR, CYP93E2, and CYP72A63; (B) bAS, CPR, and CYP93E2 as controls. (C) The structures were deduced from mass spectra. tables and figures

  10. C Peak 2 in (A) Authentic 24-OH-β-amyrin (6) Peak 14 in (A) Authentic 30-OH-β-amyrin (14) Peak 15 in (A) Authentic 11-deoxo-glycyrrhetinic acid(15) Peak 19 in (A) Peak 20 in (A) Peak 21 in (A) Peak 22 in (A) Supplementary Fig. S7 (Cont.) tables and figures

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