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ECOGEM Metabolomics: Phytochemical Investigation of Metrosideros polymorpha

ECOGEM Metabolomics: Phytochemical Investigation of Metrosideros polymorpha. Ben Clark and Swapan Pramanick College of Pharmacy, University of Hawaii at Hilo. Project Overview. Plant metabolites as markers for plant health Establish baseline levels of metabolites

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ECOGEM Metabolomics: Phytochemical Investigation of Metrosideros polymorpha

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  1. ECOGEM Metabolomics:Phytochemical Investigation of Metrosideros polymorpha Ben Clark and Swapan Pramanick College of Pharmacy, University of Hawaii at Hilo

  2. Project Overview • Plant metabolites as markers for plant health • Establish baseline levels of metabolites • How do metabolite levels change with changing environment? • Target organisms • Ohi’a lehua (Metrosideros polymorpha) • Ulu (breadfruit) (Artocarpus altilis) Ohi’a Ulu

  3. Isolation Scheme • Initial fractionation through solvent partition • Allows separation into fractions of differing polarities Ohia leaves Methanol extraction Crude extract Hexane-methanol partition Hexane fraction Methanol fraction CH2Cl2-water partition CH2Cl2 fraction Aqueous fraction butanol-water partition Butanol fraction Water fraction

  4. Isolation Strategy • Several Fractionation steps required • Use high volume techniques first, then high resolution • Different techniques for different polarities

  5. Butanol fraction • Countercurrent fractionation Countercurrent partition system Countercurrent separation of butanol solubles HPLC analysis of countercurrent fractions

  6. Dichloromethane Fraction • High volume separation by silica column • Followed by Prep TLC, HPLC • Yields terpenoids, C-methyl-flavanoids, lipids Dr. Swapan Pramanick with HPLC 1H NMR (d6-DMSO, 400 MHz) of betulinic acid

  7. Compounds Isolated • Several compounds isolated and characterized • Wide range of structure classes lipids terpenoids flavonoid glycosides C-methyl-flavonoids

  8. The Team • Faculty / Staff • Prof. Robert Borris (Team Lead) • Dr Norman Arancon, CAFNRM • Dr. Ben Clark • Dr. Swapan Pramanick • Students • Marcus Kouma • Tina MacDonald • Stephanie Ogle • Rosa Quan

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