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Microbe Control

Microbe Control. Environmental Controls Temperature Radiation Pressure pH Moisture Control Methods. Temperature. Growth Temperature Maximum Optimum Minimum Heat Cold. Radiation. Blue Light UV X-rays Gamma Rays Photoreactivation. Pressure. Obligate barophiles 10m = 1 atm

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Microbe Control

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  1. Microbe Control • Environmental Controls • Temperature • Radiation • Pressure • pH • Moisture • Control Methods

  2. Temperature • Growth Temperature • Maximum • Optimum • Minimum • Heat • Cold

  3. Radiation • Blue Light • UV • X-rays • Gamma Rays • Photoreactivation

  4. Pressure • Obligate barophiles • 10m = 1 atm • 100m = 10 atm • 1000m = 100 atm • 3000m = 300 atm • 10000m = 1000 atm • Pseudomonas bathecetes

  5. pH • Most • Marine • Commensal or parasitic • Molds vs. Bacteria

  6. Moisture • Osmosis • Hyper- vs Hypo-osmotic • Bacteria vs Molds • Food Preparation • Dried Foods • Freeze-Dried Foods • “Cured” Foods

  7. Microbe Control Methods • TERMS: • Sterilize • Kill or remove all life present • Disinfect • Kill pathogens present

  8. Microbe Control Methods • TERMS: • Germicide • Kill harmful microbes: fungicide, bacteriocide • Antiseptic • Prevents sepsis or putrefaction • Inhibits growth • Sanitize • Make safe to use or eat from

  9. Ways to Sterilize • Burning • Boiling • Autoclave (pressure cook) • Filtration • Irradiation • Chemicals

  10. Pasteurization • Internal temperature • Batch vs Flow through methods

  11. Disinfectant Action • Injure cell membrane • Change permeability • Lyse • Denature proteins

  12. Disinfectant Action • Factors Affecting Effectiveness: • Concentration • Duration of Contact • Temp., pH • Media • Microbe • Circadian Cycles

  13. Disinfectant Action • Detergents and Soaps • Acids and Bases • Alcohols • Iodine, Chlorine

  14. Disinfectant Action • Mercury • Cresols • Hydrogen peroxide (H2O2) • Formalin • Antibiotics

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