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Dream Gene

Dream Gene. Characteristics. Small (less than 1,200 base pairs) Measurable Output ( Riboswitch , Color) Functions in E. coli, but orthogonal Already Studied/or not Beneficial Pharmaceutical Biofuel Chemical C ommodities Bioremediation. Websites about Naphthalene.

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Dream Gene

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  1. Dream Gene

  2. Characteristics • Small (less than 1,200 base pairs) • Measurable Output (Riboswitch, Color) • Functions in E. coli, but orthogonal • Already Studied/or not • Beneficial • Pharmaceutical • Biofuel • Chemical Commodities • Bioremediation

  3. Websites about Naphthalene • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC106482/pdf/am003633.pdf • http://www.ncbi.nlm.nih.gov/pubmed/16804694 • http://www.ncbi.nlm.nih.gov/pubmed/16923069 • http://www.ncbi.nlm.nih.gov/pubmed/17937670 • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168525/pdf/632330.pdf • http://www.uniprot.org/uniprot/D5D8A6 • http://jb.asm.org/content/179/12/3851.full.pdf+html

  4. Naphthalene (Na+/H+ antiporterNhaC) Pros Cons A part of the sequence might get stuck between the phospholipids bilayer “2-gene operon encompassing nhaC and a small gene that was designated nhaS” Directly downstream of nt repeats • 1,401 base pairs • In E. coli • Has not been optimized • Active Transport: solute A(out) + solute B(in) = solute A(in) + solute B(out). • Blue coloring when oxidized • 7 different genes to choose from

  5. Website for other genes • http://www.ncbi.nlm.nih.gov/gene

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