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Symbols to Know for Crosses. a/a – a is the allele and / represents the two chromatids – there are two alleles for a diploid organism a b/a b – two different genes on the same chromosome a/a ; b/b – two different genes on different chromosomes ; designated separate chromosomes
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Symbols to Know for Crosses • a/a – a is the allele and / represents the two chromatids – there are two alleles for a diploid organism • a b/a b – two different genes on the same chromosome • a/a ; b/b – two different genes on different chromosomes ; designated separate chromosomes • X – symbolizes mating between two different individuals • X - symbolizes a self cross – when the hermaphrodite worms fertilize their own eggs • + is the wild type or non-mutant form of a gene
Mapping 1: Making the double mutant hermaphrodite self cross X gametes Progeny genotype Progeny phenotypes and ratios What if the genotype of the parent was + unc/+ unc?
Mapping 2: Making heterozygotes for mapping Hermaphrodite Parent genotype Male Parent genotype X Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Why might you see Dpy Unc progeny?
Mapping 3: Test Cross Hermaphrodite Parent genotype Male Parent genotype X Parental Recombinant Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios