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Authors: Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU,

COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE DE L ’ ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE. Authors:

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Authors: Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU,

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  1. COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALSETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE Authors: Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU, Dr. Gabriela MARCUS, student Adriana COJOCARU University of MedicineandPharmacy Targu-Mures ROMANIA

  2. Aim • To assess the effect of homeopatic remedies in laboratory animals in placebo controled studies • Do homeopaticremedies act through placebo effect?

  3. Material and method • Mice and rats from the bio-base (experimental station) of the University of Medicine and Pharmacy Targu-Mures • Various induced experimental pathology followed by treatment • homeopathic, • allopathic • combined, homeopathic and allopathic

  4. Experiments The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision The antitrauma effect of the remedyArnica in laboratory mice The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Homeopathic treatment of grade I burns in ratswith Apis and Belladonna

  5. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

  6. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision • Previous studies made around the world with homeopathic Arnica –contradictory results • experimental studies with Staphysagria - missing • What are the benefits in surgery? • Our hypothesis – the need of administration of the remedy earlyafter surgery

  7. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and method • 75 Wistar rats, weight 250-270 g • 5 groupsof 15 animals

  8. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodSurgery technique Step 1 - tranquilization of the rats with ether

  9. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodSurgery technique Step 2 - preparing the surgery zone by depilation and disinfection

  10. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodSurgery technique Step 3 - performing a deep incision on the external side of the thigh, following the line of the femur until the appearance of the bone

  11. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodSurgery technique Step 4 - skin suture in 3 separate points with nonresorbable silk thread

  12. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodTreatment Each rat was treated through a plastic probe, starting immediately after surgery and daily administration afterwards until the complete healing of the incision

  13. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and methodTreatment The homeopathic remedies were dissolved in water, 1 pellet in 1 ml water • Group1- control group, sugar water as placebo, • Group2- Arnica CH 7, • Group3- Arnica CH 30, • Group 4 - Staphysagria CH 7, • Group 5 - Staphysagria CH 30.

  14. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Material and method the length of the remaining incision was measured each day the number of days necessary for the complete healingwas noted for each rat

  15. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision Results The rats treated homeopathically, both remedies and both dilutions, showed a shorter healing time (15-16 days) than the placebo group (21-22 days), with a statistically significant difference (p<0,001, t-test)

  16. The antitrauma effect of the remedy Arnica in laboratory mice

  17. The antitrauma effect of the remedy Arnica in laboratory mice • 40 AKR2 white mice, • 2 groups of 20 mice: control, Arnica • Kocher clamp trauma at the level of the posterior limb • fractures • swelling • pain = protection of the affected limb, adynamia • Assesment of the variation of the dorsoplantar diameter

  18. The antitrauma effect of the remedy Arnica in laboratory mice Results • Control group • Persistent swelling more than 180 minutes • adynamia • Protection of the limb • Arnica CH7 group • Moderate swelling, gone in less than 105 minutes (p < 0,001) • Good general state • Ability to use the affected limb, even fractured • normal activity

  19. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna

  20. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedyBelladonna • Pilocarpine – parasympathomimeticeffect – salivation in mice • Atropine(Atropa Belladonna)- parasympatholyticeffect, blocking of the muscarinic receptors, imposibility for pilocarpine to produce salivation • Belladonnain homeopatic dilution – does it compensatethe atropinic intoxication?

  21. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method 80 de white mice AKR2 line divided in 4 groups: • Assessment of the salivation produced by pilocarpine (administration of pilocarpine). • Assessment of the muscarinic receptors blockade by atropine, given pilocarpine (administrationof atropine, then pilocarpine). • Assessment of supposed compensation of muscarinic blockade of atropine by homeopathic Belladonna CH7 (administrationof atropine, then Belladonna CH7, then pilocarpine). • Control group, assessment of physiologic salivation (administration of placebo).

  22. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method Step 1 – subcutaneous injection of Atropine 0,1%

  23. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method Step 2 - Belladonna CH7 given by gastric probe

  24. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method Step 3 – tranquilization with ethylic urethan intraperitoneally 1,5 g/kgc

  25. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method Step 4 - subcutaneous injection with 1% clorhidric pilocarpine

  26. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Material and method The mice were put on filter paper The area of salivary impregnation was measured each 10 minutes for 30 minutes

  27. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Results The physiologic salivation was low, nearly immeasurable on the filter paper – group 4

  28. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Results Pilocarpine produces an abundant salivation (known pharmacologic effect), assessed through the saliva spot area on the filter paper – group 1

  29. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Results Atropine given before pilocarpine prevented the salivation, which wasvery low, nearly immeasurable on the filter paper (known pharmacologic effect) – group 2

  30. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Results If homeopathic Belladonna CH 7 is given after atropine and before pilocarpine, the abundant salivation is present – group 3

  31. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Results The variation of salivationin the first 30 minutesin the 4 groups

  32. The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna Discussions • In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the ability to be stimulated by pilocarpine. • It is possible, in theory, that the action of the homeopathic remedy Belladonna CH 7 could be one of the following: • it acted on the muscarinic receptors, determining them to release the atropine molecules and become sensible again to pilocarpine stimulation; • it acted on the atropine molecules, determining them to release the receptors on which they were bound; • it acted on cellular level, determining possible alternative ways for the de processing of the stimulus represented by pilocarpine (activation of new receptors, alternative biochemical or neuroendocrine ways)

  33. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

  34. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice • Stricnina(Strychnos nux-vomica L) - adynamia, tonico-clonic convulsions, hypersensitivity to sensorial stimuli, deformation of visual and acoustic perceptions, tachycardia, tachypnea, agitation and finally death. • Diazepamul, benzodiazepină - tranquilizing, muscle-relaxant, anticonvulsant • Nux Vomica, Strychnos nux vomica seeds dilutions –used in cases of convulsions, aggravated by external stimuli, agitation, hypersensitivity to stimuli

  35. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Material and method 3 groups of 20 white mice - experimental poisoning - intraperitoneal injection of 1,5 mg/kg strychnine • Group 1 - diazepam5 mg/kg subcutaneous 1 hour before strychnine • Group 2 - Nux Vomica CH7orally by plastic probe immediatelly after strychnine • Group 3 - control (placebo), physiologic serum subcutaneous, sugar water by plastic probe

  36. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Material and method • Strychnine poisoning: • adynamia • tachycardia • tachypnea • agitation • tonico-clonic convulsions • death

  37. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Material and method We assessed: • The time until convulsions • The number of animals which had convulsions • The time until death • The number of animals which died

  38. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Results The time until convulsions • 180-390 sec Placebo group • 240-570 sec Diazepam group • 660-1080 sec homeopathic Nux Vomica group

  39. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Results • Convulsions present 100% in the Placebo and Diazepam group • 70% of mice had convulsions in the homeopathic Nux Vomica group

  40. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Results • Placebo groupmicedied in 240-610 sec • Diazepamtreated mice lived longer, 370-685 sec • Homeopathic Nux Vomica treatedmice showed the longest survival, 605-1150 sec

  41. The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Results • All the mice (100%) in the Placeboand Diazepamgroup died • Homeopatically treated mice with Nux Vomicadied in 80%

  42. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

  43. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde • Formaldehyde– mastocyt reaction - histamine • Apis - swelling, redness, pain, warmth • Hydrocortisone Hemisuccinate (HHS) - steroidal anti-inflammatory • Diclofenac - nonsteroidal anti-inflammatory

  44. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Material and method • 70 white mice – local swellingin the posterior limb by subcutaneous formaldehyde 0.5% • Dorsoplantar diameter was measured before formaldehyde and then each 15 minutes

  45. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Material and method 7 groups of 10 mice • Hydrocortisone Hemisuccinate • Diclofenac • Apis CH 7 • Apis CH 15 • Hydrocortisone Hemisuccinate and Apis CH 7 • Hydrocortisone Hemisuccinate and Apis CH 15 • Control group, placebo

  46. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Results • Local signs of inflammation • redness • swelling • motor impairment • agitation • protection of the limb

  47. Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Results Dorsoplantar diameter evolution in time(average values)

  48. Homeopathic treatment of grade I burns in ratswith Apis and Belladonna

  49. Homeopathic treatment of grade I burns in ratswith Apis and BelladonnaMaterial and method • 3 groups of 10 rats • Tranquilization with ether • Immersion of posterior left limb in hot water at 55°C for 20 seconds

  50. Material and method Grade I burns - edemaand erithema

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