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Web-based analysis and annotation tools

WIS Genome &Bioinformtics. Web-based analysis and annotation tools. Nili Avidan. The Crown Human Genome Center & The National Lab for Genomic Infrastructure Supported by the Ministry of Science. WIS Genome & Bioinformtics. WIS Genome &Bioinformtics.

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Web-based analysis and annotation tools

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  1. WIS Genome &Bioinformtics Web-based analysis and annotation tools Nili Avidan The Crown Human Genome Center & The National Lab for Genomic Infrastructure Supported by the Ministry of Science

  2. WIS Genome & Bioinformtics

  3. WIS Genome &Bioinformtics

  4. WIS Genome &Bioinformtics

  5. WIS Genome & Bioinformtics

  6. WIS Genome &Bioinformtics

  7. WIS Genome &Bioinformtics

  8. Use blast2 sequence from BLAST tool to compare the new sequence to the known cDNA in the database

  9. Use BLAST 2 sequence with blastX as program to detect AA change Your new DNA sequence AA sequence from database

  10. WIS Genome &Bioinformtics

  11. WIS Genome & Bioinformtics

  12. Kcourse.gif WIS Genome &Bioinformtics

  13. GESTALT figure of an analyzed genomic interval Blastn results of this genomic sequence WIS Genome &Bioinformtics H963 KIAA0001

  14. centromere telomere WIS Genome &Bioinformtics a TRALPUSH (+strand)b 5’ 3’ 3’ 5’ GPCR genes cluster (-strand) H963KIAA0001K1LGR P2Y12 P2Y12LGR Proscan promoter prediction 60.41 55.60 76.91 75.00 54.07 53. 05 62.34 71.18 57.33 NCBI clones AC024886 AC011103 AC020636 AC078816 KB

  15. WIS Genome &Bioinformtics mRNA sequences • Expressed Sequence Tags (ESTs): dbEST • Full mRNA sequences: RefSeq • EST clusters: UniGene • EST assemblies: • TIGR Human Gene Index • The HIB database of annotated UniGene clusters (MIPS) • AllGenes based on DoTS assemblies (U Penn.) • STACK (SANBI) • Predicted genes

  16. WIS Genome &Bioinformtics

  17. WIS Genome & Bioinformtics H89282

  18. WIS Genome &Bioinformtics

  19. WIS Genome &Bioinformtics *

  20. WIS Genome &Bioinformtics

  21. WIS Genome &Bioinformtics H89282

  22. WIS Genome &Bioinformtics

  23. WIS Genome &Bioinformtics

  24. WIS Genome &Bioinformtics MIPS assembly H11548S1 length: 1373 Contain 21 members *Predicted protein annotation TIGR assembly THC394205 length: 1373 Contain 12 members

  25. WIS Genome &Bioinformtics

  26. Multiple proteins alignment tool Your E-mail Copy/past your protein and it ortholog/homolog here

  27. MULTICLUSTAL alignment of the five GPCRs

  28. Copy/Past you protein in the box

  29. Found two known motif Histidine acid phosphatase at AA391-405 and a Zinc finger at AA1269-1292

  30. WIS Genome & Bioinformtics

  31. WIS Genome & Bioinformtics

  32. WIS Genome & Bioinformtics

  33. WIS Genome & Bioinformtics

  34. Kidney, embryonic cell line Normal prostate tissue Brain Kidney, embryonic cell line Brain, primary tumor WIS Genome &Bioinformtics Brain, normal, Post-mortem Brain, medulloblastoma

  35. Thank You!

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