1 / 12

Enzymes In Depth

Enzymes In Depth. With pH!. pH. Enzymes. Globular proteins Built from amino acids that have acidic and basic characteristics Amino acids have both a carboxylic acid (COOH) and amine (NH3) groups. Enzymes. R-COOH  C-COO -1 + H+1 R-NH3 +1  R-NH2 + H+1 Ionic bonds

hoyt-mccray
Download Presentation

Enzymes In Depth

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Enzymes In Depth With pH!

  2. pH

  3. Enzymes • Globular proteins • Built from amino acids that have acidic and basic characteristics • Amino acids have both a carboxylic acid (COOH) and amine (NH3) groups

  4. Enzymes • R-COOH  C-COO -1 + H+1 • R-NH3 +1  R-NH2 + H+1 • Ionic bonds • Increase solution pH can shift equilibrium

  5. Active sites have R groups • R groups help substrates attach to active site • Only works if the arrangement of R groups interact properly

  6. +/- • H = Hydrogen bonding • R = Van der Waals bonding

  7. At optimal pH • Enzyme attaches to substrate with 2 ionic bonds  • Groups allow for ionic bonding (caused by a transfer of H ion from COOH in the side chain of one amino acid to a NH2 group

  8. Acidic pH • Nothing will bother the NH3 + but the COO will want a H ion • pH (acid = more H ions; basic = more OH ions) • No ionic bond can form 

  9. Basic pH • COO- will not be affected • But NH3+ will lose a H • No ionic bond will form 

More Related