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GFP-Pax Y118F : -- cl. 1 -- cl. 1 cl. 2

*. 35. vector. Pax Y118F cl.1. 30. Pax Y118F cl.2. 25. 20. 15. 10. 5. 0. 100. 80. 60. 40. 20. 0. A. v-Src. Colony-forming frequency (%). FAK+/+ FAK -/-. GFP-Pax Y118F : -- cl. 1 -- cl. 1 cl. 2. FAK+/+ [Src]. FAK-/- [Src].

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GFP-Pax Y118F : -- cl. 1 -- cl. 1 cl. 2

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  1. * 35 vector PaxY118F cl.1 30 PaxY118F cl.2 25 20 15 10 5 0 100 80 60 40 20 0 A v-Src Colony-forming frequency (%) FAK+/+ FAK -/- GFP-PaxY118F: -- cl. 1 -- cl. 1 cl. 2 FAK+/+ [Src] FAK-/- [Src] Ab: GFP PaxpoY118 GAPDH Clonogenic Efficiency (%) v-Src FAK+/+ [Src] FAK-/- [Src] FAK+/+ FAK -/- 35 mock GFP-WT-Pax:- + - + 30 GFP-WT-Pax 25 Colony-forming frequency (%) 20 Ab: GFP 15 10 GAPDH 5 0 FAK+/+[v-Src] FAK-/-[v-Src] v-Src B 30 vector FAK+/+ FAK -/- 25 p120Y228F 20 Flag-p120Y228F : -- + -- + Colony-forming frequency (%) 15 Ab: FLAG p120poY228 GAPDH 10 5 0 FAK+/+ [Src] FAK-/- [Src] C v-Src 35 FAK+/+ FAK -/- 30 vector GST-ShcY239/240F: -- + -- + ShcY239/240F 25 Colony-forming frequency (%) 20 Ab: GST ShcpoY239/240 GAPDH 15 10 5 0 FAK+/+ [Src] FAK-/- [Src]

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