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Key points: summarized from chapter 14

Key points: summarized from chapter 14. RNA processing. Outline: we will focus on mRNA processing. Overview of the Central Dogma Link DNA to RNA transcription to proteintranslation What is a codon? Gene organization Introns and exons What really is a gene? Steps of pre-mRNA processing

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Key points: summarized from chapter 14

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  1. Key points: summarized from chapter 14 RNA processing

  2. Outline: we will focus on mRNA processing • Overview of the Central Dogma • Link DNA to RNA transcription to proteintranslation • What is a codon? • Gene organization • Introns and exons • What really is a gene? • Steps of pre-mRNA processing • Eukaryotes versus prokaryotes

  3. Gene Organization: Is DNA and RNA co-linear?

  4. 3 steps of mRNA processing occurs in the nucleus of eukaryotes 5’ CAP Poly-A tail Splicing

  5. The 5’Cap

  6. The Poly-A Tail

  7. Cleavage by endonuclease

  8. Basic steps of poly-Adenylation

  9. Splicing Spliceosome Requires RNA + proteins Function as enzymes Self splicing Only RNA! Function as enzymes!

  10. Spliceosome Meet the spliceosome

  11. Splicesome: 3 components are required Note some important aspects of the 5’ and 3’ ends 5’ phosphodiester of G 2’ OH of A lariat

  12. Where does splicing occurs?

  13. Self-Splicing mRNA’s

  14. Common characteristic of self-splicing mRNA’s

  15. Comments on Alternative processing methods First: What are the methods of mRNA processing discussed so far? 5’ CAP 3’ Poly A Tail Intron Splicing

  16. Alternative processing methods • Alternative splicing • Multiple 3’cleavage sites • RNA editing

  17. And alternative splicing example Thryoid BRAIN

  18. Silencing or interfering RNAs • siRNA • miRNA

  19. Onto translation chapter 15

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