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Plasmonic Sensors and Atomic Force Microscopy in Biology and Medicine Jason H. Hafner

Plasmonic Sensors and Atomic Force Microscopy in Biology and Medicine Jason H. Hafner Department of Physics & Astronomy Rice University. LSPR Refractive Index Sensitivity. ( L ocalized S urface P lasmon R esonance). E. k. B. Chemically synthesized LSPR substrates. antibodies. SAM.

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Plasmonic Sensors and Atomic Force Microscopy in Biology and Medicine Jason H. Hafner

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  1. Plasmonic Sensors and Atomic Force Microscopy in Biology and Medicine Jason H. Hafner Department of Physics & Astronomy Rice University

  2. LSPR Refractive Index Sensitivity (Localized Surface Plasmon Resonance) E k B

  3. Chemically synthesized LSPR substrates antibodies SAM gold nanorod APTES glass Clean slide NR slide EM: 50x15 nm rods at 100/um2 Spectrum of slide: NIR peak at 800 nm

  4. LSPR Immunoassay: Kinetics kon koff d Keq = kon/koff = 1.9 x 109 M-1 koff = 6.5 x 10-5 s-1 kon = 1.3 x 105 M-1s-1

  5. LSPR Immunoassay: Specificity d specific binding nonspecific binding

  6. Increased Sensitivity: Proximity A shorter SAM increases the signal rate. A smaller capture agent (aptamer) increases the signal rate for a much smaller target (thrombin, 30 kD). kon = 9140 M-1s-1 koff = 6.6 x 10-4 s-1 Keq = 1.33 x 107 M-1 C16 4.7 nm/hr 6.5 nm/hr C11 6.1 nm/hr

  7. Increased Sensitivity: Nanoparticle Shape Hybrid nanorod, nanosphere, and bipyramid substrate nanospheres nanorods LSPR shift (nm) bipyramids time (s)

  8. Experimental Setup

  9. Single Molecule LSPR Immunoassay Single particle dark field microspectroscopy under fluid flow: 50 nm Discrete blue-shifts correspond to single antigen unbinding, and occur at the correct rate:

  10. miRNA injection LSPR Assay Immunoassay: -demonstrates real time molecular analysis -cannot compete with ELISA Basic Research: -analyze biomolecular interactions -useful in “resource poor” environment -explore non-microscope single particle analysis Biomedical: -microRNA detection

  11. Plasmonic Nanobubbles Why gently heat something when you can blow it up? pulse: 0.5 ns, 0.1 J/cm2 bubble lifetime: 10 - 100 ns bubble size: 10 - 1000 nm

  12. Plasmonic Nanobubbles Why gently heat something when you can blow it up? pulse: 0.5 ns, 0.1 J/cm2 bubble lifetime: 10 - 100 ns bubble size: 10 - 1000 nm

  13. Plasmonic Nanobubbles: Cell Theranostics

  14. Cell Theranostics in vivo

  15. Plasmonic Nanobubbles: Drug Delivery Burst a liposome that contains dye and nanoparticles Fluorescence Scattering Before Bubble During Bubble After Bubble

  16. l-DNA AFM Scan contrast = topography Lift Scan contrast = charge density Electrostatic Mapping with the AFM Lift scan AFM scan Lift height Johnson et al, Langmuir19, p10007 (2003)

  17. Imaging Lipid Membranes DOPC / SM / Chol DOPC

  18. Lipid Membrane  d tr s Position Mica Mapping Lipid Membranes topography charge Lo La All lipids are zwitterionic – why the charge contrast?

  19. Plasmonic Sensors: Colleen Nehl, Katie Mayer Plasmonic Nanobubbles: Dmitri Lapotko, Dan Wagner, Cindy Farach-Carson Membrane Electrostatics: Yi Yang, Katie Mayer hafner@rice.edu

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