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stage 1. stage 2. stage 3. stage 4. stage 5. B. A. C. D. E. RA. Cell aggregation. Nestin , DAPI. F1. Neuron formation. I. K. RC2 , DAPI. G. RC2 , DAPI. b III-tub. SSEA-1 , DAPI. F2. Non-differentiated NE-4C cells. b III-tub , DAPI. H. b III-tub , RC2 , DAPI. L. RC2.
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stage 1 stage 2 stage 3 stage 4 stage 5 B A C D E RA Cell aggregation Nestin,DAPI F1 Neuron formation I K RC2,DAPI G RC2,DAPI bIII-tub SSEA-1,DAPI F2 Non-differentiated NE-4C cells bIII-tub,DAPI H bIII-tub,RC2,DAPI L RC2 merge F3 bIII-tubulin relative area field values [%] J