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Immuno-labelling for TEM

liquid nitrogen. cryo-chamber. temp. control. sectioning control. Immuno-labelling for TEM. Sample processing Fix 4% PFA Wash/Quench Harvest cells/tissue 3a. Gelatin embedding 2.3M sucrose infusion Cut ≤1mm 3 pieces Mount on cryo-stub Snap freeze liquid N 2.

ilandere-wauters
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Immuno-labelling for TEM

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  1. liquid nitrogen cryo-chamber temp. control sectioning control Immuno-labelling for TEM Sample processing Fix 4% PFA Wash/Quench Harvest cells/tissue 3a. Gelatin embedding 2.3M sucrose infusion Cut ≤1mm3 pieces Mount on cryo-stub Snap freeze liquid N2 http://www.liv.ac.uk/emunit

  2. 1. 2. 3. 4. Immuno-labelling for TEM Trim Section Pick-up Add to EM grids Grid looping Drain excess Dry Immuno-labelling MC/UA contrasting http://www.liv.ac.uk/emunit

  3. Block Wash Quench 1°Ab 5. 6. Immuno-labelling for TEM 50µl washes 5µl/grid Ab incubations Trim Section Pick-up Add to EM grids Immuno-labelling MC/UA contrasting Grid looping Drain excess Dry http://www.liv.ac.uk/emunit

  4. 7. 8. 9. Immuno-labelling for TEM Trim Section Pick-up Add to EM grids Immuno-labelling MC/UA contrasting Grid looping Drain excess Dry http://www.liv.ac.uk/emunit

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