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AP Lab 7: Genetics of Drosophila (fruit flies). Carolina. Purpose. Use fruit flies to do genetic crosses. Learn to determine the sex of fruit flies and recognize contrasting phenotypes.
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Purpose • Use fruit flies to do genetic crosses. • Learn to determine the sex of fruit flies and recognize contrasting phenotypes. • Collect data from F1 and F2 generations and analyze the results of a monohybrid, dihybrid, or sex-linked cross.
Activity A • In part A you will learn to: • observe wild-type flies • recognize male and female flies • compare the mutant phenotypes with the wild type. • http://www.phschool.com/science/biology_place/labbench/index.html
Procedure • Anesthetizing flies will be demonstrated. • Flies will be placed in petri dishes to be observed. • Draw and document important differences between phenotypes of: • Males and females • Wild types and mutants
Procedure • After you have observed the flies, return them to the correctly labeled morgue. • Make sure that if flies begin moving they are returned to the morgue quickly. • The anesthetizing agent lasts approximately 50 min.
Activity B • Setting up vials: • Obtain one vial and add 1 level measure cup of Formula 4-24 to the vial. • Add 1 level measure cup of tap water to the vial. • After it solidifies, sprinkle 4-7 grains of yeast to the culture
Procedure • Setting up F1 cross: • Record the code for the cross that you were given. • Collect 5-6 male/female pairs. (10-12 flies total) • Using Table 2: F1 Data, record sex and phenotypes for the flies you were given. • Place these flies in your vial and label with information from the lab. • Return remaining flies to the morgue.
