Dormancy of cells and organisms -strategies for survival and preservation

1. Dormancy of cells and organisms -strategies for survival and preservation NEST - # 012674 2005-2008 Acronym: Sleeping Beauty

2. Partners • Esther Lubzens (NIO) Ora Hadas, Assaf Sukenik (KLL) -IOLR –P1 • Joan Cerdà, IRTA, Barcelona, Spain – P2 • Stefan Hohmann, Goteborg University, Sweden – P3 • Melody Clark and Roger Worland, BAS, Cambridge, UK – P4 • Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – P5 • Jens Nielson, Danish Technical University, Lyngby, Denmark- P6

3. Objectives Learning from nature to developnew conceptsforcell preservationbased onmechanisms with whichmodel organismstolerate desiccation and/or remainmetabolically inactivefor long periods

4. Goals • Reveal strategies employed by different organisms that facilitate their long term survival in a dormant form • Widening the basis of our knowledge on the molecular details of strategies employed by 5model organisms • Resolve whether these strategies can be used to develop preservation (“ reversible dormancy ”) approaches at ambient temperatures for different cells and organisms * Emphasis on understanding processes that control the establishment and maintenance of dormant stages and the exit from dormancy

5. Specific goals WP 1 & 2 Discover the genes, proteins and compounds that are associated with the formationof dormant forms and exit from it in: Cyanobacteria Yeast Rotifers WP 3. Discerning the processes associated with desiccation as a survival strategy by global gene expression, proteome and metabolome profiling Arctic springtail killifish embryos

6. Specific Goals - Continued WP 4. Confirming the role of specific genes in tolerance to desiccation or dormancy by genetic analyses and genetic genetic engineering experiments Yeast Killifish Rotifers (genetically selected strains) WP 5. Discovering common and divergent strategies through synthesis of the genomic, proteomic and metabolomic data of model organisms and of data from other organisms, including plants – in silico models

7. Specific Goals - Continued • WP 6. Publicity to stir and encourage new research direction Scientific publications, scientific meetings Workshop Book • Tools: High through approaches; genomics, proteomics, metabolomics and bioinformatics

8. Partners • Ora Hadas, Assaf Sukenik (IOLR)- Cyanobacteria • Joan Cerdà, IRTA, Barcelona, Spain - Killifish • Stefan Hohmann, Goteborg University, Sweden - yeast • Melody Clark & Roger Worland, BAS, UK – Arctic sprintail • Richard Reinhardt, Max Planck Institute –Molecular Genetics, Berlin-Dahlem, Germany – Genomic and proteomic platform • Jens Nielson, Danish Technical University, Lyngby, Denmark -metabolomic platform

11. Pert Chart for SLEEPING BEAUTY