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1. 2. 3. 4. 5. 6. 7. Supplementary Figure 1. B. A. eRF3b wild type. eRF3b F66A/F75A. eRF3b F66A. eRF3b F75A. pCMV-5xMyc-. pCMV-5xMyc-. eRF3b. eRF3a. eRF3a. (kDa). (kDa). 117. 5xMyc-eRF3b. 5xMyc-eRF3b. 117. (WB: -Myc). 89. 5xMyc-eRF3a. 89. (WB: -Myc). 89.
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1 2 3 4 5 6 7 Supplementary Figure 1 B A eRF3b wild type eRF3b F66A/F75A eRF3b F66A eRF3b F75A pCMV-5xMyc- pCMV-5xMyc- eRF3b eRF3a eRF3a (kDa) (kDa) 117 5xMyc-eRF3b 5xMyc-eRF3b 117 (WB: -Myc) 89 5xMyc-eRF3a 89 (WB: -Myc) 89 Cellular eRF3 (WB: -eRF3) 117 5xMyc-eRF3a GAPDH 35 Cellular eRF3 89 (WB: -GAPDH) (WB: -eRF3) 1 2 3 4 5