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Supplementary Figure2. Cellular Localization of PHYB:GFP in csa1 mutant plants. Confocal microscope image of a root tip cell from a 8 d-old csa1 mutant plant transformed with the PHYB::GFP construct, showing the PHYB:GFP localization in nuclear speckles. Fluorescence signals were examined using a SP1 Leica confocal microscope with 63x oil-immersion lens. Excitation of GFP was at 488 nm and detection at 500 to 530 nm.