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DIO1

Supplement Figure 2. MSA of ORF-junctions of human bicistronic mRNAs in multiple organisms. DIO1. gi|89357933|ref|NM_000792.5| GGTGCTGAATTTTGGAAGTTGTACC TGA CCTTCATTTATGTTCAAATTTGACCAGTTCAA

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DIO1

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  1. Supplement Figure 2. MSA of ORF-junctions of human bicistronic mRNAs in multiple organisms DIO1 gi|89357933|ref|NM_000792.5| GGTGCTGAATTTTGGAAGTTGTACCTGACCTTCATTTATGTTCAAATTTGACCAGTTCAA gi|110825975|ref|NM_007860.3| GGTGTTGAACTTTGGCAGTTGCACCTGACCTTCATTTCTTCTCAAATTTGACCAGTTCAA gi|148277084|ref|NM_021653.3| GGTGTTGAACTTCGGCAGCTGCACCTGACCTTCATTTCTTCTCAAATTTGACCAGTTCAA gi|148277089|ref|NM_001007126.2| GGTGCTCAATTTTGGAAGTTGTACCTGACCTTCATTTCTTTTCAAATTTGACCAATTCAA gi|169791015|ref|NM_001122593.1| GGTGCTGAATTTTGGAAGTTGTACCTGACCTTCATTTATTTTCAAATTTGACCAATTCAA gi|148277085|ref|NM_001097614.1| AATCCTCAATTTTGGAAGCTGCACCTGACCTTCATTTATGTTAAAATTTGATGAGTTCAA gi|55925461|ref|NM_001007283.1| GGTTCTGAGTTTCGGAAGCTGCACCTGACCCCCGTTTCTCTACAAACTTGACGAGTTCAA * * * ** ** ** ** ******** * *** * *** **** * ***** Human Mouse Rat Dog Cow Chicken Zebrafish DIO2 Human Mouse Rat Chicken Cow Zebrafish gi|148277090|ref|NM_000793.4| TTTGCCAGCCCTGAGCGCCCACTAGTGGTCAACTTTGGCTCAGCCACTTGACCTCCTTTC gi|132566530|ref|NM_010050.2| TTTGCCAGTGCAGAGCGCCCACTGGTGGTCAACTTTGGTTCAGCCACCTGACCACCTTTC gi|132566528|ref|NM_031720.3| TTTGCCAGTGCAGAGCGCCCACTGGTGGTCAACTTTGGTTCAGCGACCTGACCACCTTTT gi|156547238|ref|NM_204114.2| TTTGCCAACTCTGAGCGGCCACTGGTGGTTAATTTTGGCTCAGCTACCTGACCACCATTC gi|156547242|ref|NM_001010992.3| TTTGCCAGCCCTGAGCGCCCACTGGTGGTCAACTTCGGCTCGGCCACTTGACCTCCTTTC gi|169790921|ref|NM_212789.3| TTTGAGTCTCCTGATCGCCCGTTAGTGGTCAACTTCGGTTCGGCCACTTGACCCCCCTTT **** * ** ** ** * ***** ** ** ** ** ** ** ***** ** **

  2. DIO3 gi|148277093|ref|NM_001362.3| TGCACCTGACCACCGTTCATGGCGCGCATGAGCGCCTTCCAGCGCCTGGTCACTAAGTAC gi|111494233|ref|NM_172119.2| TGTACCTGACCACCGTTCATGGCGCGGATGAGCGCCTTCCAGCGCCTGGTCACCAAGTAC gi|255982584|ref|NM_017210.3| TGTACCTGACCACCGTTCATGGCGCGGATGAGCGCCTTCCAGCGCCTGGTCACCAAGTAC gi|156547247|ref|NM_001010993.2| TGCACCTGACCACCGTTCATGGCGCGCATGAGCGCCTTCCAGCGCCTGGTCACCAAGTAT gi|169790978|ref|NM_001122648.1| TGCACCTGACCCCCGTTCATGGCCCGCCTGAGGTCCTTCCGGCGCCTGGCCGCGCACTTC gi|255958277|ref|NM_001164188.1| TGCACCTGACCACCGTTCATGGCGCGCATGAGCGCCTTCCACCGCCTGGTCACCAAGTAC gi|189543064:5280-6272 TGCACCTGACCCCCGTTCACGGCGCGGCTGCGCGCCTTCCAGCATCTCGCCTCCACCTTC ** ******** ******* *** ** ** * ****** * ** * * * * Human Mouse Rat Cow Chicken Dog Zebrafish GPX1 gi|41406083|ref|NM_000581.2| GGGCAAGGTACTACTTATCGAGAATGTGGCGTCCCTCTGAGGCACCACGGTCCGGGACTA gi|145275166|ref|NM_008160.5| GGGCAAGGTGCTGCTCATTGAGAATGTCGCGTCTCTCTGAGGCACCACGATCCGGGACTA gi|145275164|ref|NM_030826.3| GGGCAAGGTGCTGCTCATTGAGAATGTCGCGTCCCTCTGAGGCACCACGACCCGGGACTA gi|156602645|ref|NM_174076.3| GGGCAAGGTGCTGCTCATTGAGAACGTAGCATCGCTCTGAGGCACAACGGTGCGGGACTA gi|169403975|ref|NM_001007281.2| AGGAAAGGTTGTGCTGATTGAAAATGTGGCGTCGCTTTGAGGCACAACAGTCAGGGATTA gi|296146348|ref|NM_001179592.1| TAACAAAGTGGTTTTGATAGTCAATGTAGCATCTCATTGTGCGTTCACACCTC---AATA ** ** * * ** * ** ** ** ** * ** * ** * ** Human Mouse Rat Cow Zebrafish Yeast

  3. GPX3 gi|89903006|ref|NM_002084.3| TTTGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGTACATTGAACTGAATGCACTA gi|145275178|ref|NM_001083929.1| TTTGTCAACGTAGCCAGCTACTGAGGTCTGACAGACCAATACCTTGAACTGAATGCACTA gi|145275181|ref|NM_022525.3| TTTGTCAACGTAGCCAGCTACTGAGGTCTGACAGACCAATACCTTGAACTGAATGCACTA gi|156602650|ref|NM_174077.3| TTCGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGTACGTTGAACTGAATGCACTG gi|212276143|ref|NM_001137555.1| GTGGTCAACGTGGCCACCTACTGAGGACTGACCTTCCAGTACGTGGAACTGAATGCACTA gi|296145804|ref|NM_001179559.1| ATCGTTAATGTTGCCTCCAAATGTGGATTCACTCCTCAATACAAAGAACTAGAGGCCTTG * ** ** ** *** * * ** ** * ** ** *** ***** * ** * Human Mouse Rat Cow Zebrafish Yeast GPX4 gi|90903236|ref|NM_002085.3| CGTGTGCATCGTCACCAACGTGGCCTCCCAGTGAGGCAAGACCGAAGTAAACTACACTCA gi|90903234|ref|NM_008162.2| CGTGTGCATCGTCACCAACGTGGCCTCGCAATGAGGCAAAACTGACGTAAACTACACTCA gi|90903248|ref|NM_017165.2| CGTGTGCATCGTCACCAACGTGGCCTCGCAATGAGGCAAAACCGACGTAAACTACACTCA gi|156602651|ref|NM_174770.3| CGTGTGCATCGTCACCAATGTGGCCTCGCAATGAGGCAAGACTGACGTAAACTACACTCA gi|55925455|ref|NM_001007282.1| AGTTGTGATCATCACAAACGTTGCCTCCAAATGAGGTAAAACCCCAGTAAACTACTCTCA gi|24656766|ref|NM_168024.1| GGTGGTCCTGGTGGTGAACATCGCCTCCAAGTGCGGCCTGACCAAGAACAACTACGAGAA gi|17506886|ref|NM_060197.1| AGTTCTCATTATCGTGAACGTCGCTTCACAGTGTGGACTTACCAACAAGAATTATACACA gi|133904327|ref|NM_064677.3| AGTTCTAATCATTGTCAACGTCGCTTCACAGTGCGGACTCACCAATTCCAACTACAACCA gi|255079519|ref|XM_002503294.1| GGTTGTCATCGTCTCTAAC---------------------ACCAGGTGCCACTATGACGA ** * * ** ** * ** * Human Mouse Rat Cow Zebrafish Fruitfly Nematode Nematode Micromonas

  4. MOCS1 gi|283806691|ref|NM_005943.5| AAGAACCGGCCCATGATCCTCATCGGTGGGTGACCCATCAAGTTATTTTTGATGTTCCCC gi|161484621|ref|NM_028464.1| AAGAACCGGCCCATGATCCTCATCGGTGGGTGACCTATCAGGGTACTTTTGATGCTTCAG gi|157822764|ref|NM_001106881.1| AAGAACCGGCCCATGATCCTCATCGGGG---------------TACTTTTGATGCTTCAA gi|61888839|ref|NM_001013597.1| AAGAACCGGCCCATGATCCTCATCGGTGGGTGACCCATCAAGTTATTTTTGATGCGCCAA gi|28574487|ref|NM_176317.1| GAGAACCGGCCCATGATACTCATTGGCGGCTAGTAGA----------------------- gi|71991021|ref|NM_077210.4| CCAAATCGTCCAATGATTCTTATCGGGGGCTAA--------------------------- gi|17561209|ref|NM_074910.1| CCAAATCGTCCAATGATTCTTATCGGCGGCTAA--------------------------- ** ** ** ***** ** ** ** * Human Mouse Rat Cow Fruitfly Nematode Nematode PEG10 Human Mouse Cow Dog gi|94421474|ref|NM_015068.3| CTTCGCCGGCGGGAAACTCCCCGGCCCCGCTGTAGAGGGACCTTCAGCGACCGGGCCAGA gi|98985813|ref|NM_130877.2| ATTCGCCGCCGGGAAACTCCCCGGCCCCGCTGTAGGGGGACCTTCAGCGACAGGGCCAGA gi|289177145|ref|NM_001172518.1| CTTCGCCGGCGGGAAACTCCCCGGCCCCGCTGTAGAGGGACCTTCAGCGACCGGGCCGGA gi|289177202|ref|NM_001172543.1| CTTCGCCGGCGGGAAACTCCCCGGCCCCGCTGTAGAGGGACCTTCAGCGACCGGGCCAGA ******* ************************** *************** ***** **

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