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Mounting Media ( mountans )

Mounting Media ( mountans ). a dalah bahan yang digunakan untuk perekat pada tahap akhir pembuatan sediaan untuk diamati di bawah mikroskop. Mountants harus mempunyai ciri dan sifat : t ransparan dan tidak berwarna d apat mempenetrasi jaringan secara sempurna

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Mounting Media ( mountans )

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  1. Mounting Media (mountans) adalahbahan yang digunakanuntukperekatpadatahapakhirpembuatansediaanuntukdiamatidibawahmikroskop

  2. Mountantsharusmempunyaiciridansifat: • transparandantidakberwarna • dapatmempenetrasijaringansecarasempurna • Tidakmenimbulkanperubahanpadakomponenjaringan • Resistenterhadapkontaminasi (terutamamikroba) • Melindungiirisanjaringandarikerusakanfisikdankemis(oksidasidanperubahan pH) • Dapatlarutsempurnadenganbahandehidranatauclearing • Tidakmembentukkristaldalamjaringan, mengakibatkanpatahataumengkerutnyajaringan • tidakbereaksidengan, memudarkanataumengakibatkanbayanganpadapewarnaandanprodukpewarnaan (termasukhistokimiamenggunakanenzim, hibridisasidanimunohistokimia) • stabil

  3. Mountants • indeks bias mendekatiindeks bias protein terfiksasi (antara1.53 and 1.54)  irisanjaringannampaktransparan, berwarnajikadiwarnai. • Untuktujuantertentu (pencitraanfasekontras) digunakanmountantdenganindeks bias sedikitberbedadenganindeks bias protein jaringan.

  4. Klasifikasimountant CANADA BALSAMRI (solution) 1.523. DPX (DISTRENE, PLASTICISER, XYLENE)8-9. RI (solution) 1.523. EUPARAL5RI 1.478 at 20°C to 1.535 when solid . HIDROFOBIK : • WATER RI (1.333), • GLYCEROL RI 1.47 • POLYVINYL ALCOHOLPolyvinyl alcohol, often used as a mountant in immunofluorescence microscopy, has been recommended as an alternative for glycerine jelly.22 Adding paraphenylenediamine to the preparation is effective in retarding photo fading.23-24 HIDROFILIK

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