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1680

1930. 1680. 1960. 1515. Optics. Focus. Dr. Marvin Minsky Confocal microscope: 1957. L aser. S canning. C onfocal M icroscope. LASER. L ight A mplification by the S timulated E mission of R adiation. Dichroic Mirror. Conventional / Confocal. Conventional / Confocal.

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1680

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  1. 1930 1680 1960 1515

  2. Optics

  3. Focus

  4. Dr. Marvin Minsky Confocal microscope: 1957

  5. Laser Scanning Confocal Microscope LASER Light Amplification by the Stimulated Emission of Radiation

  6. Dichroic Mirror

  7. Conventional / Confocal

  8. Conventional / Confocal

  9. Resolution The capability of an optical system to distinguish between two objects closely adjacent

  10. Resolution The capability of an optical system to distinguish between two objects closely adjacent

  11. Resolution The capability of an optical system to distinguish between two objects closely adjacent

  12. Resolution Oversampling

  13. Resolution

  14. Deconvolution

  15. Conventional / Confocal / Biphoton

  16. Manz at al. J Microbiol Methods 2000, 40:125-34. Fig. * Tissue sections of Chondrosia reniformis after hybridization with the Bacteria specific probe EUB338 AutoDeblur from Media Cybernetics Deconvolution

  17. Frequency 255 100 25 0 255 0 Intensity Image treatment

  18. Colocalization

  19. Colocalization Seladin-1 Golgi Battista et al. Endocrinology 2009; 150: 4180 FIG 2. Immunofluorescence detection of seladin-1 in rat adult fasciculata cells Merge + Mask

  20. Colocalization Demandolx et al. J of Microscopy 1997, 185: 21 FIG 1. Double immunofluorescence labelling of fibroblast transfectants monitored by confocal microscopy. Iak MHC class II molecules are represented in green and the Ii chain is represented in red.

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