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Absorption Spectrocopy/Time-Resolved Absorption

Absorption Spectrocopy/Time-Resolved Absorption. Topic 4 Part 3 Biophysics. Time Resolved Optical Density TROD. Pump-probe. Stopped-flow. http://fig.cox.miami.edu/~cmallery/255/255enz/ecb3x28.jpg. Perturb System, measure change in absorption over time.

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Absorption Spectrocopy/Time-Resolved Absorption

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  1. Absorption Spectrocopy/Time-Resolved Absorption Topic 4 Part 3 Biophysics

  2. Time Resolved Optical DensityTROD Pump-probe Stopped-flow http://fig.cox.miami.edu/~cmallery/255/255enz/ecb3x28.jpg Perturb System, measure change in absorption over time.

  3. SVD and data analysis(eg. for Hofrichter paper) • Truncated X (or A) data matrix is fit to exponentials or other model • Get noise filtering Basis Specta Time Course of Basis Specta www.math.tau.ac.il/~nin/Courses/BioInfo04/svd.ppt

  4. Circular Dichroism and Optical Rotary Dispersion CD • CD is the differential absorption of circularly polarized light • ORD rotates linearly polarized light (and other) • Both result from chiral structures as can be used as structural probes (eg Protein 2ndary Structure) ORD http://upload.wikimedia.org/wikipedia/commons/thumb/d/d1/Circular_dichroism.png/622px-Circular_dichroism.png

  5. Polarized Absorption Arrangements can be made to measure elements directly, using PEM or using ellipsometric techniques.

  6. Ellispometric Technique • Key is crossed (or nearly crossed) polarizers as in this schematic for TRORD. • Used for protein folding (Goldbeck paper).

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