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Elettroforesi

Elettroforesi. (segue). SDS-PAGE. Pozzetti. Stacking gel. Running gel. Elettroforesi di proteine in condizioni native.

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Elettroforesi

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  1. Elettroforesi (segue)

  2. SDS-PAGE Pozzetti Stacking gel Running gel

  3. Elettroforesi di proteine in condizioni native (A) Analysis of binding of the b subunit cytoplasmic domain (bsol) to ECF1 and ECF1 (-). Mixtures of polypeptides were first analyzed by native agarose gel electrophoresis (A) through a 1% agarose gel. Lanes from left to right: I, ECF1 (-) II, bsol ; III,  IV, bsol +  ; V, bsol + ( 1-134); VI, bsol + ECF1 (-); VII,  + ECF1 (-); VIII, bsol +  + ECF1 (-); IX, ECF1 (-) + ( 1-134); X, bsol + ECF1 (-) + ( 1-134). (B) Bands were excised from the agarose gel and separated on a 10-18% gradient of polyacrylamide in SDS. Rodgers et al (1997) J. Biol. Chem. 272: 31058

  4. Visualizzazione delle proteine separate • Coomassie brilliant blue R-250 • Coomassie brilliant blue G-250 • Silver stain • Coloranti fluorescenti • Trasferimento su membrana (blotting)

  5. Coomassie R-250 G-250

  6. Reazione con formaldeide Riduzione di Ag+ Silver stain

  7. Sensibilità a confronto Comparison of the sensitivity achieved with SYPRO, silver and Coomassie brilliant blue stains. Identical SDS-polyacrylamide gels were stained with A) SYPRO Orange protein gel stain, B) SYPRO Red protein gel stain; C) silver stain and D) Coomassie brilliant blue stain, according to standard protocols.

  8. Western blot Tank Semi-dry

  9. ECL (Enhanced chemiluminescence) Luminol

  10. Risultato 1 2 3 4 5 6 Lane 1: standard pei molecolari Lane 2-6: campioni

  11. Isoelettrofocalizzazione (IEF)

  12. Elettroforesi bidimensionale (2-DE)

  13. Esempio di separazione IEF 3-10 lineare, SDS-PAGE 12.5%

  14. - Si O Elettroforesi capillare + - Effetto elettroendoosmotico

  15. Elettroforesi capillare

  16. Elettroforesi capillare (CE)

  17. CE - Esempio

  18. Sequenziamento DNA

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