1 / 19

呂凱筠 蕭光良

The Insulin-like Growth Gactor II mRNA Binding protein 1 Associates with the Gag Protein of Human Immunodeficiency Virus Type 1 and Its Overexpression Affects Virus. 呂凱筠 蕭光良. HIV Life Cycel. Nature Reviews Microbiology 5, 912-916 (2007). HIV Gag protein.

ivan
Download Presentation

呂凱筠 蕭光良

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. The Insulin-like Growth Gactor II mRNA Binding protein 1 Associates with the Gag Protein of Human Immunodeficiency Virus Type 1 and Its Overexpression Affects Virus 呂凱筠 蕭光良

  2. HIV Life Cycel

  3. Nature Reviews Microbiology 5, 912-916 (2007) HIV Gag protein www.aidsreagent.org/program_info.cfm

  4. Gag

  5. IMP1 Diminishes HIV Production Cell Supernatant BH10 FLAG-IMP1 FLAG-ΔRRM(1-2) FLAG-ΔKH(1-4) FLAG-ΔKH(3-4)

  6. IMP1 Decreases HIV Infectivity

  7. IMP1 doesn't Affect RNA Dimerization

  8. Top Bottom Pr55Gag P40 P24 TfR Pr55Gag P40 P24 TfR Pr55Gag P40 P24 TfR

  9. Nature Rev Microbiol 5:912 (2007) HIV Gag protein www.aidsreagent.org/program_info.cfm

  10. Coexpression of HIV-1 with dominant-negative proteins caused viruses to arrest at a very late stage in assembly Stalk Cell, 114:701 (2003)

  11. PABP IMP binding site in HIV • Origin: CCCAAAAAAAUUU (13-mer) ＊: CGCCAAAAAUUU (12-mer) ＊ ＊

  12. C - G G - C A - U G - C U C U A - U G - C A - U C - G C - G C G U U A C C U C A G A G C A C U G A G U C A U C A A - U A - U C - G U - A C - G A G C C G - C A - U U - A U - A G - C G - U U - A C - G U - G C - G U - A G G C A G A A A G - C U - G C - G G - C U - A U - A C - G A G U C - G G - C A - U A - U C - G G - C U - G G - U G - C G - U U - A A - U G - U C A G U - A U - A C - G G - U U - A C - G A - U C - G A G G A G C - G G - C A - U U - A C - G G G C G G - C C - G U - A C - G U - A G - C G - C A A U C A A - U G - C G - U U - A G G - C U - A U5 PBS G G A - U C - G U - G G - C G A A C C C A C G G G TAR A G U - G U - A U - A U - A C - G C - G C - G C A C G U A G G A U G A U G +200 C A +150 Poly(A) A A A A A SL1 (DIS) U - A G - C G - C U - A C - G U - A C - G SL4 C SL2 G A G SD U A C C U C G U G G U SL3 () U - A G - C U - G G - C U - A G G +250 R U - A C - G A - U +50 U5 A U G - C C - G G - C G - C +100 G U - G C - G U - A G G G C AAAAAUUUUGA AAGGAGAGA AAGCG U G U G C C C G +300 +363 +1

More Related