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Hoechst a-Tubulin Merge Figure S7. Fluorescence microscopy of CPT-treated LLCPK-1a cells. Cells were grown on glass coverslips and treated with CPT for 12 hours. Cells were then fixed. Nuclear morphology was revealed by staining with Hoechst 33342. White arrow: apoptotic body with the AMN enclosing a small nuclear fragment. Yellow arrow: large cellular fragment with an AMN.