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Methods to study molecular architecture. Microscopy - electron and light Bacterial genetics and molecular biology - identify genes necessary for synthesis and regulation Biochemistry - examine separate parts, determine chemical composition, activity Extremely powerful combination. RNA.
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Methods to study molecular architecture • Microscopy - electron and light • Bacterial genetics and molecular biology - identify genes necessary for synthesis and regulation • Biochemistry - examine separate parts, determine chemical composition, activity • Extremely powerful combination
RNA • mRNA: associated with ribosomes; being translated into protein • tRNA: transfer RNAs, charged with amino acids • rRNA: along with 56 different proteins make up the ribosome
Inclusions • poly-b-hydroxyalkanoate (PHA) or poly-b-hydroxybutyrate (PHB) • Carbon and energy sources • Glycogen (C and E sources) • Phosphates (C. diphtherae), sulfides (e-donor) • Gas vesicles (buoyancy, Cyanobacteria)