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MCB 730: Southern Blot Lab

MCB 730: Southern Blot Lab. Southern, Northern & Western blotting Flowchart Detailed Protocol. Who is this guy?. Sir Edwin Mellor Southern of course (1938- ). Inventor of the Southern blot http://en.wikipedia.org/wiki/Edwin_Southern. Characterization: Southern blot hybridization.

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MCB 730: Southern Blot Lab

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  1. MCB 730: Southern Blot Lab Southern, Northern & Western blotting Flowchart Detailed Protocol

  2. Who is this guy?

  3. Sir Edwin Mellor Southern of course (1938- ) • Inventor of the Southern blot • http://en.wikipedia.org/wiki/Edwin_Southern

  4. Characterization: Southern blot hybridization -transfer of DNA from a gel to a membrane (e.g., nitrocellulose, nylon) -developed by Edwin Southern

  5. Characterization: Northern blot hybridization X RNA X x salt X RNA -transfer of RNA from a gel to a membrane (e.g., nitrocellulose, nylon) -reveals mRNA size (and approximate protein size), tissue- and organ- specific expression, and kinetic patterns of expression

  6. Characterization: Western blotting X Protein Enzyme reaction or X React with Antibody X x Buffer; requires electric current X -transfer of protein from a gel to a membrane (e.g., nitrocellulose, nylon) -requires the use of an electric current to facilitate transfer

  7. DNA Microarrays: An Introduction

  8. Microarray Result: Much analysis to follow

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