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Bg-X-As-B. IRES *. LTR. eGFP. Amp. gag. CMVpro. RU5. U3 RU5. B = BamHI P = PvuII E = EcoRI X = XhoI A = AvaIII C = ClaI Ag = AgeI N = NotI S = SacII Sp = SplI. St = StuI Sh = SphI Bs = BspMII Ss = Sse8387I As = AsuII Ac = AccI
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Bg-X-As-B IRES* LTR eGFP Amp gag CMVpro RU5 U3 RU5 B = BamHI P = PvuII E = EcoRI X = XhoI A = AvaIII C = ClaI Ag = AgeI N = NotI S = SacII Sp = SplI St = StuI Sh = SphI Bs = BspMII Ss = Sse8387I As = AsuII Ac = AccI Hd = HinDII Sa = SalI Bg = BglII M = MfeI pAP2 (6787 bp) HA-B-P-E-X-A-C-Ag-N-S-Sp-St-Sh-Bs-Ss-As-Ac-Hd-Sa-Bg-M IRES eGFP T7 SP6 400 bp pPRIG HAa/b/c (6159/60/61 bp)