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IPM CRSP International Plant Diagnostic Network: A Gateway to IPM Implementation

IPM CRSP International Plant Diagnostic Network: A Gateway to IPM Implementation. Sally A. Miller Department of Plant Pathology. 7 th Int. IPM Symposium Memphis, TN 27 March 2012. International Plant Diagnostic Network (IPDN). Funded by USAID as part of IPM CRSP program, beginning 2005

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IPM CRSP International Plant Diagnostic Network: A Gateway to IPM Implementation

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  1. IPM CRSP International Plant Diagnostic Network: A Gateway to IPM Implementation Sally A. Miller Department of Plant Pathology 7th Int. IPM Symposium Memphis, TN 27 March 2012

  2. International Plant Diagnostic Network (IPDN) • Funded by USAID as part of IPM CRSP program, beginning 2005 • Plant disease and pest diagnostics • Network of laboratories in Africa, Asia and Central America • No regulatory or enforcement role; but communication with national plant health regulatory services is encouraged

  3. Networking to Improve Diagnostic Efficiency • Organized system of laboratories and personnel communicating with one another and working together • Hierarchical structure • Example: U.S. National Plant Diagnostic Network Central Laboratory Regional “hub” labs “Spoke” or “node” labs Communication with first responders: Farm agents, farmers, consultants, etc.

  4. Disease Diagnostic Capacity in Africa: Baseline Survey 2006-2007

  5. Identified Capacity Development Needs – EA 2008 • Additional in-depth training on high impact pest and disease diagnosis • Equipment upgrades • Better access to biotech materials and supplies • Updated pest lists • Improved local diagnostics and surveillance capacity • Better means of reaching farmers – “Test, Don’t Guess” • Improved communication and cooperation among pathologists and entomologists in AU • Access to library/reference materials • Diagnostics standardization across labs (SOPs)

  6. IPDN Approach • Introduce distance diagnostic and data management web portal • Conduct training programs • Harmonize diagnostic protocols (SOPs) • Develop and test diagnostic assays

  7. Training in Plant Diagnostics • Regional insect pest and disease diagnosis training • Include local experts • Cooperate with IPM CRSP Virus Global Theme and Regional programs • Trainees mostly drawn from IPM CRSP RPs • The Ohio State University • Advanced serological, molecular and online diagnostics

  8. Regional Hands-On Training Approaches • 20 workshops since 2006 • Horticultural crop focus • Initial stakeholder meetings (capacity assessment) + training • 466 professionals trained • Evolution of training concepts • Broad general diagnostics • Classical methods – symptoms, culturing, LFDs, squash blots (viruses), morphology • DDIS-CIMS • Focused hands-on technology training with critical disease and pest examples • Crop and technology focus • PCR, ELISA, DDIS-CIMS • SOP training

  9. IPDN Professional Programs – Phase 1

  10. Benin, 2006

  11. IPDN Professional Training– Phase II

  12. Ghana 2011: Tomato Disease and Insect Pest Diagnostics • Overviews -major diseases and pests • Bacterial diseases • Culture, HR, serology • Virus diseases • Sap transmission, serology, PCR • Insect pests and vectors identification • Fungal disease identification • Culturing, microscopy, serology • Root knot nematode identification • DDIS-CIMS training • Diagnostics and IPM implementation

  13. Diagnostics Workshop - Bangladesh • Fundamentals • PCR • Serology • Bacterial pathogen ID • Insect-transmitted virus ID • Whitefly molecular markers • Biocontrol agent ID • DDIS • Case Studies – Hands-on • Papaya mealybug • Ralstoniasolanacearum • TLCV

  14. Standard Operating Procedures • Background • Symptoms • Signs • Media recipes • Serological tests • Biochemical tests • DNA extraction • PCR protocols • Widely tested and validated

  15. Standard Operating Procedures under IPDN Development – East Africa

  16. Long-Term Training

  17. Thank you! • Ohio State University • Mark Erbaugh • Melanie Lewis Ivey • FulyaBaysal-Gurel • NagendraSubedi • Virginia Tech • Sue Tolin • Muni Muniappan • Doug Pfeiffer, Don Mullins • George Norton, Jeff Alwang • Univ. Florida • Carrie Lapaire Harmon • Tim Momol, P. Vergot • JiannongXin • UC-Davis - Bob Gilbertson • Penn State - Ed Rajotte • Makerere University – Uganda • Sam Kyamanywa, Mildred Ochwo-Ssemakula • Kenya Agricultural Res Inst • Zachary Kinyua, MonicahWaiganjo • SokoineUniv of Agriculture - TZ • AmonMaerere, DelphinaMamiro • IITA • RanajitBandyopadhyay, Fen Beed • University of Ghana-Legon • Eric Cornelius, Rodney Owusu-Darko • Agroexpertos – Guatemala • Marco Arevalo • Univ. del Valle • Margarita Palmieri • Tamil Nadu Agricultural University • Mohan Kumar, KarthiKeyan • Bangladesh Agricultural Res Inst • YousufMian, M. Rahman, S. Nahar

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