There are 3867000 breeders in 227 hatcheries in Bangladesh 1

1. Isolation and Characterization of E. coli from Day Old Chicks of a Selected Breeder Farm in Bangladesh M.A. Rashid1, 2, K.A. Chowdhury1, S.M.S. Mahmud1, T. Chowdhury3, Abdulla Al Mamun Bhuyan4, Zannat – Nahar1, S.K.Paul1,3* 1Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2Department of Livestock Services, 3International Centre for Diarrheal Disease Research (icddr,b), Dhaka, Bangladesh, 4Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Bangladesh Table 2. Total viable count (TVC) obtained from gut of DOC Results Background • We were able to isolate E. coli was from gut, liver and lungs of DOC (Table 1). • Total viable count (TVC) in gut of “A” grade chicks was 2.3×106, in “B” grade 2.6×106, in “C” grade 2.9×106, mean of which was 2.6×106 (Table 2). • The TVC of liver in “A” grade chicks was 2.6×105, in “B” grade 2.7×105, in “C” grade 2.8×105, the mean of which was 2.6×105 (Table 2). • in the lungs of “A” grade chicks was 2.2×104, in “B” grade 2.3×104 and in “C” grade was 2.5×104. The mean of which was 2.3×104 (Table 2). • Antibiotic sensitivity test revealed that E. coli were sensitive to Ciprofloxacin, Amoxicillin and Enrofloxacin, but resistant to Erythromycin and Cloxacillin. • There are 3867000 breeders in 227 hatcheries in Bangladesh1 • Bacterial diseases caused highest mortality in chicken2 • Very few attempts were taken to study the E. coli of day old chick( DOC)3,4 • E. coli of DOC is important both for DOC and the parent stock. Objectives • To determine the E.coli in vital organs like liver, lungs and gut of healthy (A grade), weak (B grade), culled (C grade) chicks obtained immediately after hatching • To determine the Total Viable Count of bacteria to show the difference in their density in different organs of the three grade of chicks • To determine the antibiotic sensitivity pattern of isolates Table 3. Biochemical characteristics of E. coli Methods • Study design: cross-sectional • Duration: April 2008 to May 2009 • Study site: laboratory of the Department of Microbiology and Hygiene, Bangladesh Agricultural University,Mymensingh • Visceral sample collected aseptically from eighteen DOC of different groups were collected from a Breeder farm of Valuka, Mymensingh • Tested the incubated sample for E.coli through competitive cultural examination and biochemical tests • Total Viable Count (TVC) was done on nutrient agar following ten-fold dilution • Lastly antibiotic sensitivity test was conducted to know their drug sensitivity and resistance pattern. E. coli growth on EMB agar media- Metallic shin E. coli growth on SS agar Table 1. Bacteria isolated from different organs of 3 batches of DOC + indicates Positive, MR = Methyl Red, - indicates Negative, VP= Voges-Proskauer Conclusions and Recommendations • From the findings of different cultural, staining, biochemical examinations it may be concluded that the isolated organism was E. coli • The load of E. coli was higher among the lower grade chicks and in gut • The E. coli we isolated during this study showed resistance to certain antibiotics which tends to recommend the proper use of antibiotics • In future more extensivestudies can beconductedtodesign a successful control estrategias A” quality chicks (Grade-A) References 1. Anonym, 2010. Farm Poultry And Livestock Survey. Bangladesh Bureau of Statistics, Statistics Division, Ministry of Planning, Government of the People’s Republic of Bangladesh. 2. Uddin MZ, Samad MA , Kabir SML, 2011. Mortality and disease status in hy-line and isa-brown strains of layer chickens reared in cage system in Bangladesh. Bangl J Vet Med, 9, 1 –16. 3. Islam A, Majumder S, Rahman A, Trisha AA, Amin R, 2012. Retrospective study of commercial poultry diseases. Eurasian J. Vet. Sci. 28, 2, 116-121. 4. Islam A, Trisha AA, Das M, Amin MR, 2009. Retrospective study of some poultry diseases at Guibandha district in Bangladesh. Bangl J Vet Med, 7, 239-247. B” quality chicks (Grade-B) E.Coli growth on Blood agar Total viable count (TVC) of E. coli C” quality chicks (Grade- c) Acknowledgements AMOX • I am grateful to Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University ,Mymensingh -2202, Bangladesh. Special thanks to Dr.Najmul haider ,Dr.Ausraful islam and Dr. Sukanta (RI of icddr,b) for their valuable suggestion to preparing my poster. ENRO CIP Antibiotic sensitivity pattern Correspondence: paul@icddrb.org