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Maze Solving Bacteria

Maze Solving Bacteria. Aim:. End. Checkpoint 1. Start site. Implementation Outline:. Goal 1 embedded with a signalling molecule Bacteria start off with receptors for this Once first bacteria reach goal, they secrete chemoattractant and an Autoinducer (AI)

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Maze Solving Bacteria

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  1. Maze Solving Bacteria

  2. Aim: End Checkpoint 1 Start site

  3. Implementation Outline: • Goal 1 embedded with a signalling molecule • Bacteria start off with receptors for this • Once first bacteria reach goal, they secrete chemoattractant and an Autoinducer (AI) • Once enough bacteria are attracted to the goal, the AI triggers a switch to chemorepellant release. • Also, receptors replaced with those for compound at goal 2. • Bacteria move off to goal 2 START Goal 1 Goal 2

  4. R0051: Promotor, lambda cI repressed Toggle Switch B0012 B0012 C0012: LacI B0034 B0034 C0051: cI B0010 B0010 Initial state: LacI is produced, cI is repressed. R0011: Promotor, repressed by LacI.

  5. AIP AgrC AgrA AgrA - P Checkpoint 1: • Checkpoint embedded with AIP • Arrival triggers AgrA - P production • This activates attractant production (cI is low) and production of the LuxI autoinducer • When enough bacteria arrive, LuxI triggers switching of the toggle (next slide) • lacI falls and repellant is produced Attractant LuxI Repellant R0051 cI repressed C0061 R0011 lacI repressed

  6. Switching: AgrA cI ComA R0051: cI Repressed R0011: LacI Repressed R0062: Activated by LuxR and HSL • Activation of cI expression should cause switching of toggle. • LacI goes low, therefore ComA (part of signalling pathway for molecule at next checkpoint) can be expressed • cI stops production of AgrA.

  7. ComX ComP ComA ComA - P Checkpoint 2: On arrival: LacI = Low, cI = high Q00400: Tet inverter LacR Promotor activated by LuxO - P (present when LuxM is low) Attractant LuxM Repellant R0011: LacI Repressed R0051 cI repressed

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