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ixr1 Δ

Supplementary Fig 1. YPD + 200 μ M tBHP. YPD. A. wt. ixr1 Δ. ixr1 Δ sml1 Δ. mec1 Δ sml1 Δ. ixr1 Δ mec1 Δ sml1 Δ. YPD + 0.025mg/L 4-NQO. YPD + 0.01mg/L 4-NQO. wt. ixr1 Δ. ixr1 Δ sml1 Δ. mec1 Δ sml1 Δ. ixr1 Δ mec1 Δ sml1 Δ. YPD + 0.005% MMS. YPD + 0.001% MMS. wt.

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ixr1 Δ

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  1. Supplementary Fig 1 YPD + 200 μM tBHP YPD A wt ixr1Δ ixr1Δsml1Δ mec1Δsml1Δ ixr1Δmec1Δsml1Δ YPD + 0.025mg/L 4-NQO YPD + 0.01mg/L 4-NQO wt ixr1Δ ixr1Δsml1Δ mec1Δsml1Δ ixr1Δmec1Δsml1Δ YPD + 0.005% MMS YPD + 0.001% MMS wt ixr1Δ ixr1Δsml1Δ mec1Δsml1Δ ixr1Δmec1Δsml1Δ Increased resistance of ixr1Δ cells to DNA damagedepends on Mec1. Due to the high sensitivity of mec1Δstrains to DNA damage, this spot assay was carried out at low concentrations of DNA damaging agents. Spot assays on YPD media with various drugs were performed using 1:10 serial dilutions (1:5 for MMS) of logarithmically growing cultures of wild-type, ixr1Δ, ixr1Δ smlΔ, mec1Δsml1Δ and mec1Δsml1Δixr1Δ strains. No significant difference in sensitivity between mec1Δsml1Δand mec1Δsml1Δixr1Δwas observed.

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